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| Description
| - Mouse major urinary proteins (MUPs) have been proposed to play a role in regulat ing the release and capture of pheromones. Here, we report affinity measurements of five recombinant urinary MUP isoforms (MUPs-I, II, VII, VIII, and IX) and on e recombinant nasal isoform (MUP-IV) for each of three pheromonal ligands, (+/- )-2-sec-butyl-4,5-dihydrothiazole (SBT), 6-hydroxy-6-methyl-3-heptanone (HMH), a nd (+/-)dehydro-exo-brevicomin (DHB). Dissociation constants for all MUP-pherom one pairs were determined by isothermal titration calorimetry, and data for SBT were corroborated by measurements of intrinsic protein fluorescence. We also rep ort the isolation of MUP-IV protein from mouse nasal extracts, in which MUP-IV m RNA has been observed previously. The affinity of each MUP isoform for SBT (Kd ~ 0.04 to 0.9 uM) is higher than that for DHB (Kd ~ 26 to 58 uM), which in turn is higher than that for HMH (Kd ~ 50 to 200 uM). Isoforms I, II, VIII, a nd IX show very similar affinities for each of the ligands.
- Mouse major urinary proteins (MUPs) have been proposed to play a role in regulat ing the release and capture of pheromones. Here, we report affinity measurements of five recombinant urinary MUP isoforms (MUPs-I, II, VII, VIII, and IX) and on e recombinant nasal isoform (MUP-IV) for each of three pheromonal ligands, (+/- )-2-sec-butyl-4,5-dihydrothiazole (SBT), 6-hydroxy-6-methyl-3-heptanone (HMH), a nd (+/-)dehydro-exo-brevicomin (DHB). Dissociation constants for all MUP-pherom one pairs were determined by isothermal titration calorimetry, and data for SBT were corroborated by measurements of intrinsic protein fluorescence. We also rep ort the isolation of MUP-IV protein from mouse nasal extracts, in which MUP-IV m RNA has been observed previously. The affinity of each MUP isoform for SBT (Kd ~ 0.04 to 0.9 uM) is higher than that for DHB (Kd ~ 26 to 58 uM), which in turn is higher than that for HMH (Kd ~ 50 to 200 uM). Isoforms I, II, VIII, a nd IX show very similar affinities for each of the ligands. (en)
- Mouse major urinary proteins (MUPs) have been proposed to play a role in regulat ing the release and capture of pheromones. Here, we report affinity measurements of five recombinant urinary MUP isoforms (MUPs-I, II, VII, VIII, and IX) and on e recombinant nasal isoform (MUP-IV) for each of three pheromonal ligands, (+/- )-2-sec-butyl-4,5-dihydrothiazole (SBT), 6-hydroxy-6-methyl-3-heptanone (HMH), a nd (+/-)dehydro-exo-brevicomin (DHB). Dissociation constants for all MUP-pherom one pairs were determined by isothermal titration calorimetry, and data for SBT were corroborated by measurements of intrinsic protein fluorescence. We also rep ort the isolation of MUP-IV protein from mouse nasal extracts, in which MUP-IV m RNA has been observed previously. The affinity of each MUP isoform for SBT (Kd ~ 0.04 to 0.9 uM) is higher than that for DHB (Kd ~ 26 to 58 uM), which in turn is higher than that for HMH (Kd ~ 50 to 200 uM). Isoforms I, II, VIII, a nd IX show very similar affinities for each of the ligands. (cs)
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| Title
| - Pheromone binding by polymorphic mouse major urinary proteins
- Pheromone binding by polymorphic mouse major urinary proteins (en)
- Pheromone binding by polymorphic mouse major urinary proteins (cs)
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| skos:prefLabel
| - Pheromone binding by polymorphic mouse major urinary proteins
- Pheromone binding by polymorphic mouse major urinary proteins (en)
- Pheromone binding by polymorphic mouse major urinary proteins (cs)
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| skos:notation
| - RIV/00216224:14310/02:00007921!RIV08-GA0-14310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/02:00007921
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Binding affinity; isothermal titration calorimetry; major urinary proteins eins; pheromones; selectivity (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Žídek, Lukáš
- Novotný, Milos V.
- Sharrow, Scott D.
- Stone, Martin J.
- Vaughn, Jeffrey L.
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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