About: Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes     Goto   Sponge   NotDistinct   Permalink

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  • The strains of Staphylococcus aureus producing TSST-1 toxin can induce human disease called Toxic Shock Syndrom (TSS).Production of staphylococcal toxins is based on the function of genes, which are usually localised in chromosome of host bacterium,but sometimes they can be a part of prophage genome.Temperate bacteriophages of S.aureus can move genes by transduction and/or mediate lysogenic conversion of bacterial cells,which leads to increased ability of toxin production, usually of enterotoxins A and E. In this study 25 strains of S.aureus were analysed for presence of prophages of the serological groups A,and F. The presence of prophages was determined using hybridisation by means of specific probes to Sma I and Hin dIII DNA restriction patterns of S.aureus strains.Probes were prepared from genomic sequences of phages 77 (serological group F),53 (serological group B)and 3A(serological group A). Positive signal in Sma I restriction patterns was found in 92 %strains when probe 77A was used. On the ot
  • The strains of Staphylococcus aureus producing TSST-1 toxin can induce human disease called Toxic Shock Syndrom (TSS).Production of staphylococcal toxins is based on the function of genes, which are usually localised in chromosome of host bacterium,but sometimes they can be a part of prophage genome.Temperate bacteriophages of S.aureus can move genes by transduction and/or mediate lysogenic conversion of bacterial cells,which leads to increased ability of toxin production, usually of enterotoxins A and E. In this study 25 strains of S.aureus were analysed for presence of prophages of the serological groups A,and F. The presence of prophages was determined using hybridisation by means of specific probes to Sma I and Hin dIII DNA restriction patterns of S.aureus strains.Probes were prepared from genomic sequences of phages 77 (serological group F),53 (serological group B)and 3A(serological group A). Positive signal in Sma I restriction patterns was found in 92 %strains when probe 77A was used. On the ot (en)
  • The strains of Staphylococcus aureus producing TSST-1 toxin can induce human disease called Toxic Shock Syndrom (TSS).Production of staphylococcal toxins is based on the function of genes, which are usually localised in chromosome of host bacterium,but sometimes they can be a part of prophage genome.Temperate bacteriophages of S.aureus can move genes by transduction and/or mediate lysogenic conversion of bacterial cells,which leads to increased ability of toxin production, usually of enterotoxins A and E. In this study 25 strains of S.aureus were analysed for presence of prophages of the serological groups A,and F. The presence of prophages was determined using hybridisation by means of specific probes to Sma I and Hin dIII DNA restriction patterns of S.aureus strains.Probes were prepared from genomic sequences of phages 77 (serological group F),53 (serological group B)and 3A(serological group A). Positive signal in Sma I restriction patterns was found in 92 %strains when probe 77A was used. On the ot (cs)
Title
  • Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes
  • Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes (en)
  • Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes (cs)
skos:prefLabel
  • Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes
  • Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes (en)
  • Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes (cs)
skos:notation
  • RIV/00216224:14310/01:00007143!RIV08-MSM-14310___
http://linked.open.../vavai/riv/strany
  • 279
http://linked.open...avai/riv/aktivita
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  • Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika
  • 4
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http://linked.open...aciTvurceVysledku
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  • 677320
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  • RIV/00216224:14310/01:00007143
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  • Staphylococcus aureus; TSST-1 toxin; Toxic Shock Syndrom; molecular identification; prophages; lysogeny (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [C015B84521D9]
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  • Scripta medica
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 74
http://linked.open...iv/tvurceVysledku
  • Doškař, Jiří
  • Hrstka, Roman
  • Pantůček, Roman
  • Růžičková, Vladislava
  • Petráš, Petr
http://linked.open...n/vavai/riv/zamer
issn
  • 1211-3395
number of pages
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  • 14310
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