About: Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Species Staphylococcus aureus belongs to clinically important gram-positive cocci. Some S. aureus strains produce TSST-1 toxin, which causes human disease called Toxic Shock Syndrom (TSS). Two forms of TSS were differentiated: the first type occurs in connection with menses, the second one is formed of cases, when TSS is a complication of another staphylococcal disease, for exampte food poisoning. Data about abiGty of S. aureus strains to produce TSST-1 toxin are clearly different. Therefore, we analyzed genomic DNAs of 26 S. aureus strains isolated from patients in the Czech Republic in connection with TSS. Staphylococcal isolates were analyzed by PCR using specific oligonucleotide primers to confirm presence of specific sequences of tst gene. To determine genetic variability, we performed macrorestriction analysis using PFGE, which enables determination of 20 different PFGE types. Strains were divided into 8 PFGE groups (ET1-ET8) according to Smal restriction patteros based on the similarity le Species Staphylococcus aureus belongs to clinically important gram-positive cocci. Some S. aureus strains produce TSST-1 toxin, which causes human disease called Toxic Shock Syndrom (TSS). Two forms of TSS were differentiated: the first type occurs in connection with menses, the second one is formed of cases, when TSS is a complication of another staphylococcal disease, for exampte food poisoning. Data about abiGty of S. aureus strains to produce TSST-1 toxin are clearly different. Therefore, we analyzed genomic DNAs of 26 S. aureus strains isolated from patients in the Czech Republic in connection with TSS. Staphylococcal isolates were analyzed by PCR using specific oligonucleotide primers to confirm presence of specific sequences of tst gene. To determine genetic variability, we performed macrorestriction analysis using PFGE, which enables determination of 20 different PFGE types. Strains were divided into 8 PFGE groups (ET1-ET8) according to Smal restriction patteros based on the similarity le (en)
Title	Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic (en)
skos:prefLabel	Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic (en)
skos:notation	RIV/00216224:14310/01:00005395!RIV/2002/MSM/143102/N
http://linked.open.../vavai/riv/strany	25
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 143100008)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Hrstka, Roman Doškař, Jiří Růžičková, Vladislava Pantůček, Roman
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	681115
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/01:00005395
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic (en)
http://linked.open.../riv/klicoveSlovo	Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic
http://linked.open...ontrolniKodProRIV	[EDC0BE5BBF95]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	V. Pracovní setkání biochemiků a molekulárních biologů
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Hrstka, Roman Pantůček, Roman Růžičková, Vladislava Petráš, Petr
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2001-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100008
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Masarykova univerzita v Brně
https://schema.org/isbn	80-210-2538-7
http://localhost/t...ganizacniJednotka	14310

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