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Description
  • The polymerase chain reaction (PCR) is a suitable method which enables a rapid identification of microorganisms in foods. However, the presence of inhibitors in the food samples often complicates the identification of the target microorganisms using PCR. The aim of our work was to study the elimination of the inhibitor influence on the identification of target microorganisms in milk and other foods using PCR. As a model microorganism, Salmonella was chosen. Various procedures of the preparation of DNA matrix for PCR were used: pure DNA prepared using the phenol extraction method, boiled cell lysateswith and without immunomagnetic separation of cells. Sensitivity of the PCR reaction was followed in each procedure.
  • The polymerase chain reaction (PCR) is a suitable method which enables a rapid identification of microorganisms in foods. However, the presence of inhibitors in the food samples often complicates the identification of the target microorganisms using PCR. The aim of our work was to study the elimination of the inhibitor influence on the identification of target microorganisms in milk and other foods using PCR. As a model microorganism, Salmonella was chosen. Various procedures of the preparation of DNA matrix for PCR were used: pure DNA prepared using the phenol extraction method, boiled cell lysateswith and without immunomagnetic separation of cells. Sensitivity of the PCR reaction was followed in each procedure. (en)
Title
  • PCR inhibitors in foodstuffs
  • PCR inhibitors in foodstuffs (en)
skos:prefLabel
  • PCR inhibitors in foodstuffs
  • PCR inhibitors in foodstuffs (en)
skos:notation
  • RIV/00216224:14310/00:00003591!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany
  • 44
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/98/1231)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
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  • 721269
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  • RIV/00216224:14310/00:00003591
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • PCR inhibitors in foodstuffs (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [6FB2FD74CAFD]
http://linked.open...v/mistoKonaniAkce
  • Lednice na Moravě
http://linked.open...i/riv/mistoVydani
  • Lednice na Moravě
http://linked.open...i/riv/nazevZdroje
  • EuroConference- Modern Analytical Methods for Food and Beverage Authentication
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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  • Rittich, Bohuslav
  • Španová, Alena
  • Lenfeld, Jiří
  • Beneš, Milan
  • Čechová, Leona
  • Poborská, Pavlína
  • Škapová, Denisa
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Mendelova zemědělská a lesnická univerzita v Brně
http://localhost/t...ganizacniJednotka
  • 14310
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