Attributes | Values |
---|
rdf:type
| |
Description
| - In present study two types of cell lines, HCT8 and HT29, were used for an in vitro cultivation of the gastric parasite Cryptosporidium muris. Our observations were carried out after 24, 48 and 72 DPI using combined approach of light, electron and confocal laser scanning microscopy. So far, we succeed to detect free sporozoites with typically prolonged apical ends that seemed to search for appropriate host cell/infection site, few structures closely resembling full or already emptied cryptosporidian parasitophorous sacs, and free merozoites especially in HT29 cell line. Evidently, invading sporozoites preferred newly formed round cells or gaps in a discontinuous layer characteristic for young cell cultures. Interestingly, however, unexcysted oocysts of C. muris were found to be completely or partially enveloped by projections of individual host cells after 24 DPI in both cell lines.
- In present study two types of cell lines, HCT8 and HT29, were used for an in vitro cultivation of the gastric parasite Cryptosporidium muris. Our observations were carried out after 24, 48 and 72 DPI using combined approach of light, electron and confocal laser scanning microscopy. So far, we succeed to detect free sporozoites with typically prolonged apical ends that seemed to search for appropriate host cell/infection site, few structures closely resembling full or already emptied cryptosporidian parasitophorous sacs, and free merozoites especially in HT29 cell line. Evidently, invading sporozoites preferred newly formed round cells or gaps in a discontinuous layer characteristic for young cell cultures. Interestingly, however, unexcysted oocysts of C. muris were found to be completely or partially enveloped by projections of individual host cells after 24 DPI in both cell lines. (en)
|
Title
| - Responses of cell lines to the real and simulated inoculations with Cryptosporidium muris oocysts.
- Responses of cell lines to the real and simulated inoculations with Cryptosporidium muris oocysts. (en)
|
skos:prefLabel
| - Responses of cell lines to the real and simulated inoculations with Cryptosporidium muris oocysts.
- Responses of cell lines to the real and simulated inoculations with Cryptosporidium muris oocysts. (en)
|
skos:notation
| - RIV/00216224:14230/14:00073879!RIV15-MSM-14230___
|
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| - P(GAP505/11/1163), P(GPP506/10/P372), S
|
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14230/14:00073879
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - in vitro cultivation; cell lines; oocysts of C. muris; polystyrene microspheres (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...iv/tvurceVysledku
| - Valigurová, Andrea
- Ilgová, Jana
- Melicherová, Janka
|
http://localhost/t...ganizacniJednotka
| |