About: Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.

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About: Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two strains of human fungal pathogen Cryptococus neoformans and three strains of oportunistic pathogen Aureobasidium pullulans were examined by rhodamine-phalloidin and DAPI staining after paraformaldehyde fixation and Triton X-100 treatment to identify actin cytoskeleton structures and nuclei.Uninuclear C. neoformans cells revealed actin cables oriented to developing bud, actin patches accumulated at growing bud and actin ring in the neck region between mother and daughter cells. In contrast, opportunistic pathogen A.pullulans did not reveal actin cables or patches, while actin rings were seen in multinuclear cells.However, all cells revealed diffuse F-actin cytoplasmic staining, accumulated at tips of young hyphae that may represent simple actin cytoskeleton enabling variable morphogenetic changes. Occurence of multinuclear cells in absence of actin cables supports our hypothesis on the role of actin cables in positioning of cell nucleus. Two strains of human fungal pathogen Cryptococus neoformans and three strains of oportunistic pathogen Aureobasidium pullulans were examined by rhodamine-phalloidin and DAPI staining after paraformaldehyde fixation and Triton X-100 treatment to identify actin cytoskeleton structures and nuclei.Uninuclear C. neoformans cells revealed actin cables oriented to developing bud, actin patches accumulated at growing bud and actin ring in the neck region between mother and daughter cells. In contrast, opportunistic pathogen A.pullulans did not reveal actin cables or patches, while actin rings were seen in multinuclear cells.However, all cells revealed diffuse F-actin cytoplasmic staining, accumulated at tips of young hyphae that may represent simple actin cytoskeleton enabling variable morphogenetic changes. Occurence of multinuclear cells in absence of actin cables supports our hypothesis on the role of actin cables in positioning of cell nucleus. (en)
Title	Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans. Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans. (en)
skos:prefLabel	Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans. Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans. (en)
skos:notation	RIV/00216224:14110/99:00003238!RIV/2002/GA0/141102/N
http://linked.open.../vavai/riv/strany	38
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA204/97/1150)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Gabriel, Miroslav Svoboda, Augustin Kopecká, Marie
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	734311
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/99:00003238
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	actin cytoskeleton, human pathogenic fungi, Cryptococcus neoformans, Aureobasidium pullulans (en)
http://linked.open.../riv/klicoveSlovo	human pathogenic fungi Cryptococcus neoformans Aureobasidium pullulans actin cytoskeleton
http://linked.open...ontrolniKodProRIV	[3D5DF16984FA]
http://linked.open...v/mistoKonaniAkce	Bratislava
http://linked.open...i/riv/mistoVydani	Bratislava
http://linked.open...i/riv/nazevZdroje	20th International Specialized Symposium on Yeasts
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The cell wall and morphogenesis in yeast cells: a role for the cytoskeleton
http://linked.open...UplatneniVysledku	1999
http://linked.open...iv/tvurceVysledku	Kopecká, Marie Svoboda, Augustin Gabriel, Miroslav Takeo, Kanji
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	1999-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Czech and Slovak Yeast Commission of the Czechoslovak Society for Microbiology
http://localhost/t...ganizacniJednotka	14110

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