About: Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Leptospira spp. are spirochete bacteria comprising both pathogenic and free-living species. The saprophyte L. biflexa is a model bacterium for studying leptospiral biology due to relative ease of culturing and genetic manipulation. In this study, we constructed a library of 4,996 random transposon mutants in L. biflexa. We screened the library for increased susceptibility to the DNA intercalating agent, ethidium bromide (EtBr), in order to identify genetic determinants that reduce L. biflexa susceptibility to antimicrobial agents. By phenotypic screening, using subinhibitory EtBr concentrations, we identified 29 genes that, when disrupted via transposon insertion, led to increased sensitivity of the bacteria to EtBr. At the functional level, these genes could be categorized by function as follows: regulation and signaling (n = 11), transport (n = 6), membrane structure (n = 5), stress response (n = 2), DNA damage repair (n = 1), and other processes (n = 3), while 1 gene had no predicted function. Leptospira spp. are spirochete bacteria comprising both pathogenic and free-living species. The saprophyte L. biflexa is a model bacterium for studying leptospiral biology due to relative ease of culturing and genetic manipulation. In this study, we constructed a library of 4,996 random transposon mutants in L. biflexa. We screened the library for increased susceptibility to the DNA intercalating agent, ethidium bromide (EtBr), in order to identify genetic determinants that reduce L. biflexa susceptibility to antimicrobial agents. By phenotypic screening, using subinhibitory EtBr concentrations, we identified 29 genes that, when disrupted via transposon insertion, led to increased sensitivity of the bacteria to EtBr. At the functional level, these genes could be categorized by function as follows: regulation and signaling (n = 11), transport (n = 6), membrane structure (n = 5), stress response (n = 2), DNA damage repair (n = 1), and other processes (n = 3), while 1 gene had no predicted function. (en)
Title	Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance (en)
skos:prefLabel	Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance Screening of a Leptospira biflexa Mutant Library To Identify Genes Involved in Ethidium Bromide Tolerance (en)
skos:notation	RIV/00216224:14110/14:00078632!RIV15-MSM-14110___
http://linked.open...avai/riv/aktivita	I
http://linked.open...avai/riv/aktivity	I
http://linked.open...iv/cisloPeriodika	19
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Pětrošová, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	43948
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/14:00078632
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	GRAM-NEGATIVE BACTERIA; ACYL-CARRIER PROTEIN; MEDIATED MULTIDRUG-RESISTANCE; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; EFFLUX PUMPS; IN-VITRO; ERYTHROMYCIN RESISTANCE (en)
http://linked.open.../riv/klicoveSlovo	ACYL-CARRIER PROTEIN EFFLUX PUMPS ERYTHROMYCIN RESISTANCE GRAM-NEGATIVE BACTERIA ESCHERICHIA-COLI IN-VITRO STAPHYLOCOCCUS-AUREUS PSEUDOMONAS-AERUGINOSA BACILLUS-SUBTILIS MEDIATED MULTIDRUG-RESISTANCE
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C5E06B09E938]
http://linked.open...i/riv/nazevZdroje	Applied and Environmental Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	80
http://linked.open...iv/tvurceVysledku	Pětrošová, Helena Picardeau, Mathieu
http://linked.open...ain/vavai/riv/wos	000341887100024
issn	0099-2240
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1128/AEM.01619-14
http://localhost/t...ganizacniJednotka	14110

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