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  • The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001).
  • The aim of this study was to use digital holographic microscopy (DHM) in human sperm imaging and compare quantitative phase contrast of sperm heads in normozoospermia (NZ) and oligoasthenozoospermia (OAT). DHM spermatozoa imaging and repeated quantitative phase shift evaluation were used. Five NZ and 5 OAT samples were examined. Semen samples were examined by semen analysis and processed for DHM. Main outcome measures were maximum phase shift value of the sperm heads. Differences of the phase shift and in NZ and OAT samples were statistically tested. In NZ samples median phase shifts were in the range 2.72–3.21 rad and 2.00–2.15 in OAT samples. Differences among individual samples were statistically significant (p<0.001) in both groups. Median phase shift according to sperm count was 2.90 rad in NZ samples and 2.00 rad in OAT samples. This difference was statistically significant (p<0.001). (en)
Title
  • Digital holographic microscopy in human sperm imaging
  • Digital holographic microscopy in human sperm imaging (en)
skos:prefLabel
  • Digital holographic microscopy in human sperm imaging
  • Digital holographic microscopy in human sperm imaging (en)
skos:notation
  • RIV/00216224:14110/11:00056979!RIV12-MZ0-14110___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(NS9661)
http://linked.open...iv/cisloPeriodika
  • 8
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 194637
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/11:00056979
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  • Digital holographic microscopy; Sperm imaging; Spermatozoon; Male infertility; Chromatin integrity (en)
http://linked.open.../riv/klicoveSlovo
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  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [339603F7A539]
http://linked.open...i/riv/nazevZdroje
  • Journal of Assisted Reproduction and Genetics
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  • 28
http://linked.open...iv/tvurceVysledku
  • Crha, Igor
  • Huser, Martin
  • Pohanka, Michal
  • Ventruba, Pavel
  • Žáková, Jana
  • Lousová, Eva
http://linked.open...ain/vavai/riv/wos
  • 000294734900011
issn
  • 1058-0468
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s10815-011-9584-y
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  • 14110
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