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  • The aim of this study was to evolve an effective procedure for the sperm lysis, obtention of intracellular content and subsequent assesment of Hc concentration and, in consequence, determine the correlations between blood plasma and seminal fluid Hc concentration, spermiogram parameters and DNA injury. Firstly, we used the method of repeated freezing and melting according to Ebisch et al., 2006 for the sperm lysis. Because the microscopic control of the samples didn’t show any markable sperm injury, we projected and compared other methods of the cell lysis – ultrasound, melting in ultrasound and freezing 10 times, methanol, distilled water, Triton X-100 solution, and lysis solution + 1% Triton X-100 solution. We used the method of high-performance liquid chromatography with fluorescence detection (HPLCFD) for the determination of Hc concentration and comet analysis for the assesment of DNA injury.
  • The aim of this study was to evolve an effective procedure for the sperm lysis, obtention of intracellular content and subsequent assesment of Hc concentration and, in consequence, determine the correlations between blood plasma and seminal fluid Hc concentration, spermiogram parameters and DNA injury. Firstly, we used the method of repeated freezing and melting according to Ebisch et al., 2006 for the sperm lysis. Because the microscopic control of the samples didn’t show any markable sperm injury, we projected and compared other methods of the cell lysis – ultrasound, melting in ultrasound and freezing 10 times, methanol, distilled water, Triton X-100 solution, and lysis solution + 1% Triton X-100 solution. We used the method of high-performance liquid chromatography with fluorescence detection (HPLCFD) for the determination of Hc concentration and comet analysis for the assesment of DNA injury. (en)
Title
  • Assesment of intracellular homocysteine concentration in sperms
  • Assesment of intracellular homocysteine concentration in sperms (en)
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  • Assesment of intracellular homocysteine concentration in sperms
  • Assesment of intracellular homocysteine concentration in sperms (en)
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  • RIV/00216224:14110/11:00053108!RIV12-MSM-14110___
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  • RIV/00216224:14110/11:00053108
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  • intracellular homocysteine sperms (en)
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  • Crha, Igor
  • Králíková, Michaela
  • Lousová, E.
  • Matejovičová, Milena
  • Melounová, Jitka
  • Ventruba, Pavel
  • Vodová, M.
  • Žáková, Jana
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  • 14110
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