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  • Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.
  • Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented. (en)
Title
  • Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart
  • Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart (en)
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  • Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart
  • Experimental Methods for Simultaneous Measurement of Action Potentials and Electrograms in Isolated Heart (en)
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  • RIV/00216224:14110/10:00044615!RIV11-GA0-14110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA102/07/1473), P(GD102/09/H083), P(GP102/07/P521), V, Z(MSM0021622402), Z(MSM0021630513)
http://linked.open...iv/cisloPeriodika
  • Suppl 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 258314
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14110/10:00044615
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Voltage-Sensitive Dye; Motion Artifact; Ischemia; Calcium (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [576C68F63460]
http://linked.open...i/riv/nazevZdroje
  • Physiological Research
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • vol. 59
http://linked.open...iv/tvurceVysledku
  • Kolářová, Jana
  • Provazník, Ivo
  • Fialová, Kateřina
  • Janoušek, Oto
  • Nováková, Marie
http://linked.open...ain/vavai/riv/wos
  • 000279049100011
http://linked.open...n/vavai/riv/zamer
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  • 0862-8408
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  • 14110
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