About: Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Myocardial ischemia and reperfusion phenomena have been studied repeatedly in various models. The aim of this study was to verify preconditioning model in guinea pig and rabbit Langendorff-perfused hearts loaded with voltage-sensitive dye (VSD) di-4-ANEPPS which we use to record monophasic action potentials (MAPs) by optical method. The heart was perfused according to Langendorff at constant pressure (80mmHg) with Krebs Henseleit solution. Each experiment consisted of heart isolation, control perfusion, dye loading, washout and MAPs recording. Rabbit hearts loaded with VSD tolerated ischemia of longer duration without severe alterations of electrogram. Although the procedure of dye loading affects electrophysiological properties of the myocardium, in case of rabbit heart it is negligible (it is quite resistant to side effects of VSDs). In guinea pig mycardium, more cautiousness is needed. Myocardial ischemia and reperfusion phenomena have been studied repeatedly in various models. The aim of this study was to verify preconditioning model in guinea pig and rabbit Langendorff-perfused hearts loaded with voltage-sensitive dye (VSD) di-4-ANEPPS which we use to record monophasic action potentials (MAPs) by optical method. The heart was perfused according to Langendorff at constant pressure (80mmHg) with Krebs Henseleit solution. Each experiment consisted of heart isolation, control perfusion, dye loading, washout and MAPs recording. Rabbit hearts loaded with VSD tolerated ischemia of longer duration without severe alterations of electrogram. Although the procedure of dye loading affects electrophysiological properties of the myocardium, in case of rabbit heart it is negligible (it is quite resistant to side effects of VSDs). In guinea pig mycardium, more cautiousness is needed. (en)
Title	Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS (en)
skos:prefLabel	Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS Model of Preconditioning in Guinea Pig and Rabbit Isolated Hearts Loaded with Voltage-Sensitive Dye Di-4-ANEPPS (en)
skos:notation	RIV/00216224:14110/09:00029526!RIV11-GA0-14110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA102/07/1473), Z(MSM0021622402)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Nováková, Marie Fialová, Kateřina
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	326686
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/09:00029526
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	preconditioning; isolated heart; voltage-sensitive dye; ischemia; reperfusion (en)
http://linked.open.../riv/klicoveSlovo	ischemia preconditioning reperfusion isolated heart voltage-sensitive dye
http://linked.open...ontrolniKodProRIV	[F651F2D6568B]
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Optical Methods for Registration of Heart Electrical Potentials and Calcium Concentration with Laser Stabilization
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Kolářová, Jana Provazník, Ivo Fialová, Kateřina Nováková, Marie
http://linked.open...n/vavai/riv/zamer	Early diagnostics and treatment of cardiovascular diseases
http://localhost/t...ganizacniJednotka	14110

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