About: Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes

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About: Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Background: Quaternary benzo[c]phenanthridine alkaloids (QBAs) are naturally occurring compounds isolated from plants of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. In addition to wide biological activities, they are attractive for their fluorescence. We observed interesting fluorescent characteristics of QBAs - macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) and chelerythrine (CHE) upon interacting with living cells. Methods: Water stock solutions of the alkaloids (10-100 mg/ml) were added to intact cells and upon brief incubation the alkaloids stain the cells. Human cell lines HL60 (human promyelocytic leukemia), HeLa (human cervix adenocarcinoma) and LEP (human lung fibroblasts) and piglet blood were used in experiments. Blood cells were stained with MA in a combination with FITC-conjugated anti CD45 surface marker antibody. Cells were analysed by fluorescence microscopy and by flow cytometry. Background: Quaternary benzo[c]phenanthridine alkaloids (QBAs) are naturally occurring compounds isolated from plants of Fumariaceae, Papaveraceae, Ranunculaceae and Rutaceae families. In addition to wide biological activities, they are attractive for their fluorescence. We observed interesting fluorescent characteristics of QBAs - macarpine (MA), sanguirubine (SR), chelirubine (CHR), sanguilutine (SL), chelilutine (CHL), sanguinarine (SA) and chelerythrine (CHE) upon interacting with living cells. Methods: Water stock solutions of the alkaloids (10-100 mg/ml) were added to intact cells and upon brief incubation the alkaloids stain the cells. Human cell lines HL60 (human promyelocytic leukemia), HeLa (human cervix adenocarcinoma) and LEP (human lung fibroblasts) and piglet blood were used in experiments. Blood cells were stained with MA in a combination with FITC-conjugated anti CD45 surface marker antibody. Cells were analysed by fluorescence microscopy and by flow cytometry. (en)
Title	Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes (en)
skos:prefLabel	Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes (en)
skos:notation	RIV/00216224:14110/07:00020661!RIV10-GA0-14110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/04/0017), P(LC06077), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Táborská, Eva Slaninová, Iva Slanina, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	446200
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/07:00020661
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	benzophenanthridine alkaloids; cell-permeant DNA dye; chelirubine; flow cytometry; fluorescence microscopy; DNA content; cell cycle; macarpine; multicolor analysis; sanguinarine (en)
http://linked.open.../riv/klicoveSlovo	macarpine flow cytometry cell-permeant DNA dye chelirubine multicolor analysis cell cycle DNA content fluorescence microscopy sanguinarine benzophenanthridine alkaloids
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[FC02A962C750]
http://linked.open...i/riv/nazevZdroje	Cytometry Part A
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Plant sources of benzophenanthridine alkaloids and effects of these alkaloids on normal and cancer cells Center of Chemical Genetics
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	71A
http://linked.open...iv/tvurceVysledku	Slaninová, Iva Táborská, Eva Slanina, Jiří
http://linked.open...ain/vavai/riv/wos	000249187800011
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
issn	1552-4922
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14110
is http://linked.open...avai/riv/vysledek of	Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes Quaternary Benzo[c]phenanthridine Alkaloids - Novel Cell Permeant and Red Fluorescing DNA Probes

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