About: Surface immobilized protein multilayers for cell seeding

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The adhesion and growth of mouse embryonic stem cells line D3 were tested in polystyrene culture dishes coated with the multilayer assemblies containing various cell-adhesive proteins such as gelatin, collagen IV, and laminin or polycations polylysine and poly(ethyleneimine). The cells were seeded in complete serum-containing media or in serum-free media and in the presence (non-differentiated) and/or absence (differentiated) of leukemia inhibitory factor. The cells grew best on surfaces coated with gelatin and collagen IV assemblies. There were no significant differences in the growth of the non-differentiated and differentiated cells in complete serum-containing media. When seeded in serum-free media, non-differentiated cells grew better than the differentiated ones. The layer-by-layer deposition appears to be a practicable technique by which scaffolds for tissue engineering can be coated with biomolecular assemblies tailored to specific cells and applications. The adhesion and growth of mouse embryonic stem cells line D3 were tested in polystyrene culture dishes coated with the multilayer assemblies containing various cell-adhesive proteins such as gelatin, collagen IV, and laminin or polycations polylysine and poly(ethyleneimine). The cells were seeded in complete serum-containing media or in serum-free media and in the presence (non-differentiated) and/or absence (differentiated) of leukemia inhibitory factor. The cells grew best on surfaces coated with gelatin and collagen IV assemblies. There were no significant differences in the growth of the non-differentiated and differentiated cells in complete serum-containing media. When seeded in serum-free media, non-differentiated cells grew better than the differentiated ones. The layer-by-layer deposition appears to be a practicable technique by which scaffolds for tissue engineering can be coated with biomolecular assemblies tailored to specific cells and applications. (en)
Title	Surface immobilized protein multilayers for cell seeding Surface immobilized protein multilayers for cell seeding (en)
skos:prefLabel	Surface immobilized protein multilayers for cell seeding Surface immobilized protein multilayers for cell seeding (en)
skos:notation	RIV/00216224:14110/05:00028930!RIV10-GA0-14110___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA102/03/0633), P(GA203/02/1326), P(LN00A065)
http://linked.open...iv/cisloPeriodika	17
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pacherník, Jiří Dvořák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	545478
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/05:00028930
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MOUSE EMBRYOS; BIOSENSORS; ALBUMIN; HEPARIN; INVITRO; PLASMA (en)
http://linked.open.../riv/klicoveSlovo	PLASMA ALBUMIN BIOSENSORS HEPARIN INVITRO MOUSE EMBRYOS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[078EA4B44F93]
http://linked.open...i/riv/nazevZdroje	Langmuir
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Cell Therapy and Tissue Repair Controlled immobilization of interfacing biomolecular assemblies Optical multisensors for detection of foodborne pathogens
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	21
http://linked.open...iv/tvurceVysledku	Dvořák, Petr Pacherník, Jiří Brynda, E. Houska, M. Pientka, Z.
http://linked.open...ain/vavai/riv/wos	000231389200037
issn	0743-7463
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14110
is http://linked.open...avai/riv/vysledek of	Surface immobilized protein multilayers for cell seeding

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