About: Discrete Fourier transform-based analysis of HeLa cell microtubules after ultrasonic exposure

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cytoskeletal structures can be affected by ultrasound. We exposed HeLa cells to continuous ultrasound (1 MHz, 1 and 2 W.cm-2, 10 min. at 37 C). The microtubules were detected by the monoclonal antibody TU-01/SwAM/FITC, observed in a fluorescence microscope and photographed digitally. The images were processed by FFT magic software. The structure analysis is based on frequency domain filtering using discrete Fourier transform. We tried to design filters to extract information describing best the structural changes. The properties of the filter can be enhanced by direction filtering, i.e., extraction of a symmetric angular segment in the frequency domain centred on a zero frequency. The final image is a normalised sum of inverse FFTs of such segmented spectra. The method yielded the ratio of the area of microtubules to the total cell area. Assuming that the image background intensity is constant, we can use thresholding to detect areas occupied by the cells. Cytoskeletal structures can be affected by ultrasound. We exposed HeLa cells to continuous ultrasound (1 MHz, 1 and 2 W.cm-2, 10 min. at 37 C). The microtubules were detected by the monoclonal antibody TU-01/SwAM/FITC, observed in a fluorescence microscope and photographed digitally. The images were processed by FFT magic software. The structure analysis is based on frequency domain filtering using discrete Fourier transform. We tried to design filters to extract information describing best the structural changes. The properties of the filter can be enhanced by direction filtering, i.e., extraction of a symmetric angular segment in the frequency domain centred on a zero frequency. The final image is a normalised sum of inverse FFTs of such segmented spectra. The method yielded the ratio of the area of microtubules to the total cell area. Assuming that the image background intensity is constant, we can use thresholding to detect areas occupied by the cells. (en)
Title	Discrete Fourier transform-based analysis of HeLa cell microtubules after ultrasonic exposure Discrete Fourier transform-based analysis of HeLa cell microtubules after ultrasonic exposure (en)
skos:prefLabel	Discrete Fourier transform-based analysis of HeLa cell microtubules after ultrasonic exposure Discrete Fourier transform-based analysis of HeLa cell microtubules after ultrasonic exposure (en)
skos:notation	RIV/00216224:14110/05:00012839!RIV10-GA0-14110___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA301/01/1102)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bečvářová, Pavlína Mornstein, Vojtěch Škorpíková, Jiřina Janisch, Roman
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	518239
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/05:00012839
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytoskeleton; microtubules; immunofluorescence; FFT analysis; ultrasound; image analysis (en)
http://linked.open.../riv/klicoveSlovo	ultrasound image analysis FFT analysis cytoskeleton immunofluorescence microtubules
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[559579FA0DC9]
http://linked.open...i/riv/nazevZdroje	Microscopy Reasearch Technique
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Influencing the cytoskeleton of normal and tumour cells by physical factors and substances with anti-tumour activity
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	68
http://linked.open...iv/tvurceVysledku	Mornstein, Vojtěch Škorpíková, Jiřina Janisch, Roman Bečvářová, Pavlína Novy, Jindřich
issn	1059-910X
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	14110
is http://linked.open...avai/riv/vysledek of	Discrete Fourier transform-based analysis of HeLa cell microtubules after ultrasonic exposure

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