About: Osmotic shock affects cell wall and cytoskeleton in Saccharomyces cerevisiae

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Hyperosmotic growth medium containing 1 M KCl, 1 M mannitol, and/or 1 M glycerol caused cessation of yeast growth for about 2 h and thereafter the growth resumed at almost the original rate. Fluorescent patches on the inner surface of cell walls stained with Calcofluor white was observed. The patches gradually disappeared in buds formed in hyperosmotic medium. Freeze-etched replicas of osmotically stressed cells revealed deep plasma membrane invaginations filled from the periplasmic side with amorphous cell-wall material. The rate of incorporation of D-[U-14C]glucose into the individual cell wall polysaccharides during osmotic shock followed the growth kinetics. No differences in the composition of the cell walls from osmotically stressed yeast and those from the control cells was found. Microtubules disappeared and actin patches were present in both mother cell and bud. After 2 - 3 h in hyperosmotic medium, both microtubules and microfilaments regenerated to their original polarized forms. Strains o Hyperosmotic growth medium containing 1 M KCl, 1 M mannitol, and/or 1 M glycerol caused cessation of yeast growth for about 2 h and thereafter the growth resumed at almost the original rate. Fluorescent patches on the inner surface of cell walls stained with Calcofluor white was observed. The patches gradually disappeared in buds formed in hyperosmotic medium. Freeze-etched replicas of osmotically stressed cells revealed deep plasma membrane invaginations filled from the periplasmic side with amorphous cell-wall material. The rate of incorporation of D-[U-14C]glucose into the individual cell wall polysaccharides during osmotic shock followed the growth kinetics. No differences in the composition of the cell walls from osmotically stressed yeast and those from the control cells was found. Microtubules disappeared and actin patches were present in both mother cell and bud. After 2 - 3 h in hyperosmotic medium, both microtubules and microfilaments regenerated to their original polarized forms. Strains o (en)
Title	Osmotic shock affects cell wall and cytoskeleton in Saccharomyces cerevisiae Osmotic shock affects cell wall and cytoskeleton in Saccharomyces cerevisiae (en)
skos:prefLabel	Osmotic shock affects cell wall and cytoskeleton in Saccharomyces cerevisiae Osmotic shock affects cell wall and cytoskeleton in Saccharomyces cerevisiae (en)
skos:notation	RIV/00216224:14110/00:00003449!RIV/2002/GA0/141102/N
http://linked.open.../vavai/riv/strany	231
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA204/00/0394), P(GA204/99/D025)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Slaninová, Iva Svoboda, Augustin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	720878
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/00:00003449
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytoskeleton, cell wall, osmotic shock, yeasts (en)
http://linked.open.../riv/klicoveSlovo	yeasts cell wall cytoskeleton osmotic shock
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[EF3CBD3D3FA3]
http://linked.open...i/riv/nazevZdroje	Cellular and Molecular Biology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The yeast cell wall as an extracellular matrix: cytoskeleton and cell wall functions The role of cytoskeleton and cell wall in the cell polarization
http://linked.open...UplatneniVysledku	2000
http://linked.open...v/svazekPeriodika	46
http://linked.open...iv/tvurceVysledku	Slaninová, Iva Farkaš, Vladimír Svoboda, Augustin Šesták, Sergej
issn	0145-5680
number of pages	1 (xsd:int)
http://localhost/t...ganizacniJednotka	14110

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