About: The effect of Chelidonius majus alkaloids on tumor and yeast cells

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About: The effect of Chelidonius majus alkaloids on tumor and yeast cells Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect Four quarternary benzo(c)phenanthridine alkaloids, i.e., chelerythrine, chelilutine, sanguinarine and sanguilutine and two quaternary protoberberine alkaloids berberine and koptisine, were examined for their effects on HeLa cell line (human cervix carcinoma cells) and yeasts S. cerevisiae and Sch. japonicus var. versatilis. The agent concentrations ranged from 1 to 100 mg/ml. Cytotoxicity by neutral red (NR) uptake assay using 96-well plates was tested. In vivo antimicrotubular effect by indirect immunofluorescence of tubulin was tested. ID50 was assessed: sanguinarine ID50 = 0,8 mg/ml, sanguilutine ID50 = 8,3 mg/ml, chelerythrine ID50 = 6,2 mg/ml , chelilutine ID50 = 5,2 mg/ml, coptisine ID50 = 2,6 mg/ml and berberine ID50 =10,0 mg/ml. Alkaloids at lower concentrations (5 to 10 mg/ml) produced a decrease in microtubule numbers, particularly at the cell periphery. The strongest effect was observed in sanguinarine which showed microtubule rearangement at the concentration 0,1 mg/ml and toxic effect (en)
Title	The effect of Chelidonius majus alkaloids on tumor and yeast cells (en) Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky (cs)
skos:prefLabel	The effect of Chelidonius majus alkaloids on tumor and yeast cells (en) Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky Vliv alkaloidů Chelidonium majus na nádorové buňky a kvasinky (cs)
skos:notation	RIV/00216224:14110/00:00003448!RIV/2002/GA0/141102/N
http://linked.open.../vavai/riv/strany	97
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA204/99/D025)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Táborská, Eva Slaninová, Iva Bochořáková, Hana Slanina, Jiří
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	730932
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/00:00003448
http://linked.open...riv/jazykVysledku	cze - čeština
http://linked.open.../riv/klicovaSlova	benzophenenthridine alkaloids, cytotoxicity, cytoskeleton, (en)
http://linked.open.../riv/klicoveSlovo	cytotoxicity cytoskeleton benzophenenthridine alkaloids
http://linked.open...ontrolniKodProRIV	[1846AE7025F9]
http://linked.open...v/mistoKonaniAkce	Cěské Budějovice
http://linked.open...i/riv/mistoVydani	Cěské Budějovice
http://linked.open...i/riv/nazevZdroje	Cells II
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of cytoskeleton and cell wall in the cell polarization
http://linked.open...UplatneniVysledku	2000
http://linked.open...iv/tvurceVysledku	Slaninová, Iva Táborská, Eva Slanina, Jiří Bochořáková, Hana
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2000-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	KOPP Publishing
http://localhost/t...ganizacniJednotka	14110

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