About: 5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes     Goto   Sponge   NotDistinct   Permalink

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Description
  • Several oligothymidylates containing various ratios of phosphodiester and isopolar 5'-hydroxyphosphonate, 5'-O-methylphosphonate and 3'-O-methylphosphonate internucleotide linkages were examined with respect to their hybridization properties with oligoriboadenylates and their ability to induce RNA cleavage by ribonuclease H (RNase H). The results demonstrated that the increasing number of 5'-hydroxyphosphonate or 5'-O-methylphosphonate units in antisense oligonucleotides (AOs) significantly stabilizes the heteroduplexes, whereas 3'-O-methylphosphonate AOs cause strong destabilization of the heteroduplexes. Only the heteroduplexes with 5'-O-methylphosphonate units in the antisense strand exhibited a significant increase in Escherichia coli RNase H cleavage activity by up to 3-fold (depending on the ratio of phosphodiester and phosphonate linkages) in comparison with the natural heteroduplex. A similar increase in RNase H cleavage activity was also observed for heteroduplexes composed of miRNA191 and complementary AOs containing 5'-O-methylphosphonate units. We propose for this type of AOs, working via the RNase H mechanism, the abbreviation MEPNA (MEthylPhosphonate Nucleic Acid).
  • Several oligothymidylates containing various ratios of phosphodiester and isopolar 5'-hydroxyphosphonate, 5'-O-methylphosphonate and 3'-O-methylphosphonate internucleotide linkages were examined with respect to their hybridization properties with oligoriboadenylates and their ability to induce RNA cleavage by ribonuclease H (RNase H). The results demonstrated that the increasing number of 5'-hydroxyphosphonate or 5'-O-methylphosphonate units in antisense oligonucleotides (AOs) significantly stabilizes the heteroduplexes, whereas 3'-O-methylphosphonate AOs cause strong destabilization of the heteroduplexes. Only the heteroduplexes with 5'-O-methylphosphonate units in the antisense strand exhibited a significant increase in Escherichia coli RNase H cleavage activity by up to 3-fold (depending on the ratio of phosphodiester and phosphonate linkages) in comparison with the natural heteroduplex. A similar increase in RNase H cleavage activity was also observed for heteroduplexes composed of miRNA191 and complementary AOs containing 5'-O-methylphosphonate units. We propose for this type of AOs, working via the RNase H mechanism, the abbreviation MEPNA (MEthylPhosphonate Nucleic Acid). (en)
Title
  • 5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes
  • 5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes (en)
skos:prefLabel
  • 5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes
  • 5 '-O-Methylphosphonate nucleic acids-new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes (en)
skos:notation
  • RIV/00216208:11320/14:10287787!RIV15-MSM-11320___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GA13-26526S), P(GA203/09/0820)
http://linked.open...iv/cisloPeriodika
  • 42
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 58314
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11320/14:10287787
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • hybrid duplexes; cleavage rate; RNase H; Escherichia coli; modified DNAs; nucleic acids; 5'-O-Methylphosphonate (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [B93C2FBFF547]
http://linked.open...i/riv/nazevZdroje
  • Nucleic Acids Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 2014
http://linked.open...iv/tvurceVysledku
  • Novák, Pavel
  • Rosenberg, Ivan
  • Štěpánek, Josef
  • Barvík, Ivan
  • Homola, Jiří
  • Rejman, Dominik
  • Liboska, Radek
  • Pav, Ondrej
  • Sipova, Hana
  • Petrova, Magdalena
  • Rosenbergova, Sarka
  • Simak, Ondrej
  • Springer, Tomas
http://linked.open...ain/vavai/riv/wos
  • 000336092300060
issn
  • 0305-1048
number of pages
http://bibframe.org/vocab/doi
  • 10.1093/nar/gku125
http://localhost/t...ganizacniJednotka
  • 11320
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