About: Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions

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About: Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1007/s10863-012-9458-8
Description	The fluorescent dye 3,3'-dipropylthiadicarbocyanine, diS-C-3(3), is a suitable probe to monitor real changes of plasma membrane potential in yeast cells which are too small for direct membrane potential measurements with microelectrodes. A method presented in this paper makes it possible to convert changes of equilibrium diS-C-3(3) fluorescence spectra, measured in yeast cell suspensions under certain defined conditions, into underlying membrane potential differences, scaled in the units of millivolts. Spectral analysis of synchronously scanned diS-C-3(3) fluorescence allows to assess the amount of dye accumulated in cells without otherwise necessary sample taking and following separation of cells from the medium. Moreover, membrane potential changes can be quantified without demanding calibration protocols. The applicability of this approach was demonstrated on the depolarization of Rhodotorula glutinis yeast cells upon acidification of cell suspensions and/or by increasing extracellular K+ concentration. The fluorescent dye 3,3'-dipropylthiadicarbocyanine, diS-C-3(3), is a suitable probe to monitor real changes of plasma membrane potential in yeast cells which are too small for direct membrane potential measurements with microelectrodes. A method presented in this paper makes it possible to convert changes of equilibrium diS-C-3(3) fluorescence spectra, measured in yeast cell suspensions under certain defined conditions, into underlying membrane potential differences, scaled in the units of millivolts. Spectral analysis of synchronously scanned diS-C-3(3) fluorescence allows to assess the amount of dye accumulated in cells without otherwise necessary sample taking and following separation of cells from the medium. Moreover, membrane potential changes can be quantified without demanding calibration protocols. The applicability of this approach was demonstrated on the depolarization of Rhodotorula glutinis yeast cells upon acidification of cell suspensions and/or by increasing extracellular K+ concentration. (en)
Title	Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions (en)
skos:prefLabel	Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions (en)
skos:notation	RIV/00216208:11320/12:10131297!RIV13-GA0-11320___
http://linked.open...avai/predkladatel	Matematicko-fyzikální fakulta
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP205/10/1121), Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Gášková, Dana Plášek, Jaromír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Matematicko-fyzikální fakulta
http://linked.open...dnocenehoVysledku	151663
http://linked.open...ai/riv/idVysledku	RIV/00216208:11320/12:10131297
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Spectral analysis; Real changes of; Plasma membrane potential; Fluorescent probe; Yeast (en)
http://linked.open.../riv/klicoveSlovo	Spectral analysis Yeast Fluorescent probe Plasma membrane potential Real changes of
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[669A8A2AC797]
http://linked.open...i/riv/nazevZdroje	Journal of Bioenergetics and Biomembranes
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Biophysical method of monitoring the activity of multidrug resistance systems and membrane potential changes in yeast cells
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	44
http://linked.open...iv/tvurceVysledku	Gášková, Dana Plášek, Jaromír Ludwig, Jost Hoefer, Milan Lichtenberg-Frate, Hella
http://linked.open...ain/vavai/riv/wos	000308965000004
http://linked.open...n/vavai/riv/zamer	Fyzika molekulárních, makromolekulárních a biologických systémů
issn	0145-479X
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10863-012-9458-8
http://localhost/t...ganizacniJednotka	11320
is http://linked.open...avai/riv/vysledek of	Monitoring of real changes of plasma membrane potential by diS-C-3(3) fluorescence in yeast cell suspensions

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