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Description
  • Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell.
  • Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell. (en)
Title
  • Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution
  • Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution (en)
skos:prefLabel
  • Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution
  • Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution (en)
skos:notation
  • RIV/00216208:11320/10:10070564!RIV11-MSM-11320___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM0021620835)
http://linked.open...iv/cisloPeriodika
  • 3-4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 259806
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11320/10:10070564
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cellular uptake; microspectrofluorometry; porphyrin; oligonucleotide; Fluorescence lifetime (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [51206013413F]
http://linked.open...i/riv/nazevZdroje
  • SPECTROSCOPY-AN INTERNATIONAL JOURNAL
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 24
http://linked.open...iv/tvurceVysledku
  • Kočišová, Eva
  • Mojzeš, Peter
  • Praus, Petr
  • Sureau, Franck
  • Turpin, Pierre-Yves
  • Štěpánek, Josef
http://linked.open...ain/vavai/riv/wos
  • 000281667400019
http://linked.open...n/vavai/riv/zamer
issn
  • 0712-4813
number of pages
http://localhost/t...ganizacniJednotka
  • 11320
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