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| Description
| - There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on cell tolerance to alkali-metal cations and on membrane potential was studied. All three sodium transporters were found to participate in the maintenance of cell Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescence potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarises the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarisation of the plasma membrane and consequently increased cell sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+ antiporter could play a role in the homeostatic regulation of plasma membrane potential in yeast cells.
- There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on cell tolerance to alkali-metal cations and on membrane potential was studied. All three sodium transporters were found to participate in the maintenance of cell Na+, Li+, K+ and Cs+ homeostasis. Measurements of the distribution of a fluorescence potentiometric probe (diS-C3(3) assay) in cell suspensions showed that the lack of all three transporters depolarises the plasma membrane. The overexpression of the Na+,K+/H+ antiporter Nha1 resulted in the hyperpolarisation of the plasma membrane and consequently increased cell sensitivity to Cs+, Tl+ and hygromycin B. This is the first evidence that the activity of a Na+,K+/H+ antiporter could play a role in the homeostatic regulation of plasma membrane potential in yeast cells. (en)
- U Saccharomyces cerevisiae existují tři různé transportní systémy sodíku (Ena1-4p, Nha1p, Nhx1p). Byl studován vliv jejich nepřítomnosti na toleranci buněk vůči alkalickým kovovým iontům a na membránový potenciál. Bylo zjištěno, že všechny tři transportéry sodíku se podílejí na udržování homeostáze Na+, Li+, K+ a Cs+. Měření distribuce fluorescenční potenciometrické sondy (diS-C3(3) test) v buněčné suspenzi ukázala, že nedostatek všech tří transportérů depolarizuje plazmatickou membránu. Overexprese Na+,K+/H+ antiporteru Nha1 má za následek hyperpolarizaci plazmatické membrány a následné zvýšení citlivosti buněk k Cs+, Tl+ a hygromycinu B. Jedná se o první důkaz toho, že aktivita Na+,K+/H+ antiporteru by mohla hrát roli při homeostatické regulaci plazmatického membránového potenciálu u kvasinkových buněk. (cs)
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| Title
| - The Na+,K+/H+ antiporter Nha1 influences plasma membrane potential of Saccharomyces cerevisiae
- Na+,K+/H+ antiporter Nha1 ovlivňuje plazmatický membránový potenciál u Saccharomyces cerevisiae (cs)
- The Na+,K+/H+ antiporter Nha1 influences plasma membrane potential of Saccharomyces cerevisiae (en)
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| skos:prefLabel
| - The Na+,K+/H+ antiporter Nha1 influences plasma membrane potential of Saccharomyces cerevisiae
- Na+,K+/H+ antiporter Nha1 ovlivňuje plazmatický membránový potenciál u Saccharomyces cerevisiae (cs)
- The Na+,K+/H+ antiporter Nha1 influences plasma membrane potential of Saccharomyces cerevisiae (en)
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| skos:notation
| - RIV/00216208:11320/06:00002295!RIV07-MSM-11320___
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| http://linked.open.../vavai/riv/strany
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| - RIV/00216208:11320/06:00002295
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - K+/H+; antiporter; influences; plasma; membrane; potential; Saccharomyces; cerevisiae (en)
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