About: CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation

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About: CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.4161/rna.29441
Description	Histone acetylation modulates alternative splicing of several hundred genes. Here, we tested the role of the histone acetyltransferase p300 in alternative splicing and showed that knockdown of p300 promotes inclusion of the fibronectin (FN1) alternative EDB exon. p300 associates with CRE sites in the promoter via the CREB transcription factor. We created mini-gene reporters driven by an artificial promoter containing CRE sites. Both deletion and mutation of the CRE site affected EDB alternative splicing in the same manner as p300 knockdown. Next we showed that p300 controls histone H4 acetylation along the FN1 gene. Consistently, p300 depletion and CRE deletion/mutation both reduced histone H4 acetylation on mini-gene reporters. Finally, we provide evidence that the effect of CRE inactivation on H4 acetylation and alternative splicing is counteracted by the inhibition of histone deacetylases. Together, these data suggest that histone acetylation could be one of the mechanisms how promoter and promoter binding proteins influence alternative splicing. Histone acetylation modulates alternative splicing of several hundred genes. Here, we tested the role of the histone acetyltransferase p300 in alternative splicing and showed that knockdown of p300 promotes inclusion of the fibronectin (FN1) alternative EDB exon. p300 associates with CRE sites in the promoter via the CREB transcription factor. We created mini-gene reporters driven by an artificial promoter containing CRE sites. Both deletion and mutation of the CRE site affected EDB alternative splicing in the same manner as p300 knockdown. Next we showed that p300 controls histone H4 acetylation along the FN1 gene. Consistently, p300 depletion and CRE deletion/mutation both reduced histone H4 acetylation on mini-gene reporters. Finally, we provide evidence that the effect of CRE inactivation on H4 acetylation and alternative splicing is counteracted by the inhibition of histone deacetylases. Together, these data suggest that histone acetylation could be one of the mechanisms how promoter and promoter binding proteins influence alternative splicing. (en)
Title	CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation (en)
skos:prefLabel	CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation (en)
skos:notation	RIV/00216208:11310/14:10285589!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(GBP305/12/G034), S
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Dušková, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	9133
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10285589
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	promoter; histone acetylation; p300; fibronectin; alternative splicing (en)
http://linked.open.../riv/klicoveSlovo	p300 promoter histone acetylation alternative splicing fibronectin
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E60A47261BDF]
http://linked.open...i/riv/nazevZdroje	RNA Biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for RNA Biology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Stanek, David Hnilicova, Jarmila Kozáková, Eva
http://linked.open...ain/vavai/riv/wos	000342901600013
issn	1547-6286
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.4161/rna.29441
http://localhost/t...ganizacniJednotka	11310

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