About: Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_23
Description	The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation. The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation. (en)
Title	Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery (en)
skos:prefLabel	Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery (en)
skos:notation	RIV/00216208:11310/14:10173621!RIV15-MSM-11310___
http://linked.open...avai/riv/aktivita	I Z
http://linked.open...avai/riv/aktivity	I, Z(MSM0021620858)
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hála, Michal Cvrčková, Fatima
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	19001
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/14:10173621
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	screeening; cDNA library; phenotype rescue; complementation; inducible expression system; heterologous gene expression; Saccharomyces cerevisiae (en)
http://linked.open.../riv/klicoveSlovo	cDNA library heterologous gene expression phenotype rescue screeening inducible expression system Saccharomyces cerevisiae complementation
http://linked.open...ontrolniKodProRIV	[E261D4E5DF9D]
http://linked.open...i/riv/mistoVydani	New York
http://linked.open...vEdiceCisloSvazku	Methods in molecular biology, 1080
http://linked.open...i/riv/nazevZdroje	Methods in molecular biology (Clifton, N.J.)
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...v/pocetStranKnihy	285 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2014
http://linked.open...iv/tvurceVysledku	Hála, Michal Cvrčková, Fatima
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
number of pages	16 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-643-6_23
http://purl.org/ne...btex#hasPublisher	Springer-Verlag
https://schema.org/isbn	978-1-62703-643-6
http://localhost/t...ganizacniJednotka	11310

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