About: DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)     Goto   Sponge   NotDistinct   Permalink

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Description
  • Frustulia was used as a model genus to test four candidate markers (D1-D2 LSU rDNA, V4-SSU rDNA, rbcL-3 P, and COI-5 P) for DNA barcoding of diatoms. The Frustulia strains included in the study were primarily isolated from Europe and New Zealand. In agreement with previous reports, the results suggest that a dual-locus barcode comprising a partial sequence of the large ribosomal subunit (LSU) and a partial sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) is the best option for DNA barcoding of diatoms. Unfortunately, this approach is limited by the lack of a distinct barcode gap for these markers. Overall, the results of this study suggest that Frustulia species cannot currently be resolved without the use of additional non-molecular evidence. Effective use of DNA barcoding will require a better understanding of the mechanisms that generate and maintain genetic diversity in diatoms.
  • Frustulia was used as a model genus to test four candidate markers (D1-D2 LSU rDNA, V4-SSU rDNA, rbcL-3 P, and COI-5 P) for DNA barcoding of diatoms. The Frustulia strains included in the study were primarily isolated from Europe and New Zealand. In agreement with previous reports, the results suggest that a dual-locus barcode comprising a partial sequence of the large ribosomal subunit (LSU) and a partial sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) is the best option for DNA barcoding of diatoms. Unfortunately, this approach is limited by the lack of a distinct barcode gap for these markers. Overall, the results of this study suggest that Frustulia species cannot currently be resolved without the use of additional non-molecular evidence. Effective use of DNA barcoding will require a better understanding of the mechanisms that generate and maintain genetic diversity in diatoms. (en)
Title
  • DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)
  • DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae) (en)
skos:prefLabel
  • DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)
  • DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae) (en)
skos:notation
  • RIV/00216208:11310/13:10194130!RIV14-GA0-11310___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • I, P(GP13-07210P), S
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  • 142
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  • 70157
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  • RIV/00216208:11310/13:10194130
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  • COI-5 P; V4 SSU; rbcL-3 P; D1-D2 LSU; DNA barcoding; Frustulia; diatom (en)
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http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
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  • [A7E3AD0CBF12]
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  • Nova Hedwigia
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  • Neuveden
http://linked.open...iv/tvurceVysledku
  • Urbánková, Pavla
  • Veselá, Jana
http://linked.open...ain/vavai/riv/wos
  • 000325381600013
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  • 0029-5035
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  • 11310
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