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rdf:type
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Description
| - Frustulia was used as a model genus to test four candidate markers (D1-D2 LSU rDNA, V4-SSU rDNA, rbcL-3 P, and COI-5 P) for DNA barcoding of diatoms. The Frustulia strains included in the study were primarily isolated from Europe and New Zealand. In agreement with previous reports, the results suggest that a dual-locus barcode comprising a partial sequence of the large ribosomal subunit (LSU) and a partial sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) is the best option for DNA barcoding of diatoms. Unfortunately, this approach is limited by the lack of a distinct barcode gap for these markers. Overall, the results of this study suggest that Frustulia species cannot currently be resolved without the use of additional non-molecular evidence. Effective use of DNA barcoding will require a better understanding of the mechanisms that generate and maintain genetic diversity in diatoms.
- Frustulia was used as a model genus to test four candidate markers (D1-D2 LSU rDNA, V4-SSU rDNA, rbcL-3 P, and COI-5 P) for DNA barcoding of diatoms. The Frustulia strains included in the study were primarily isolated from Europe and New Zealand. In agreement with previous reports, the results suggest that a dual-locus barcode comprising a partial sequence of the large ribosomal subunit (LSU) and a partial sequence of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) is the best option for DNA barcoding of diatoms. Unfortunately, this approach is limited by the lack of a distinct barcode gap for these markers. Overall, the results of this study suggest that Frustulia species cannot currently be resolved without the use of additional non-molecular evidence. Effective use of DNA barcoding will require a better understanding of the mechanisms that generate and maintain genetic diversity in diatoms. (en)
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Title
| - DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)
- DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae) (en)
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skos:prefLabel
| - DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae)
- DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae) (en)
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skos:notation
| - RIV/00216208:11310/13:10194130!RIV14-GA0-11310___
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11310/13:10194130
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - COI-5 P; V4 SSU; rbcL-3 P; D1-D2 LSU; DNA barcoding; Frustulia; diatom (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Urbánková, Pavla
- Veselá, Jana
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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