About: Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases     Goto   Sponge   NotDistinct   Permalink

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Description
  • Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three-potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
  • Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three-potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications. (en)
Title
  • Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases
  • Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases (en)
skos:prefLabel
  • Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases
  • Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases (en)
skos:notation
  • RIV/00216208:11310/13:10192950!RIV14-MSM-11310___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GBP206/12/G151), P(IAA400040901)
http://linked.open...iv/cisloPeriodika
  • 38
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 63106
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  • RIV/00216208:11310/13:10192950
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • voltammetry; sequencing; nucleoside triphosphates; electrochemistry; DNA polymerase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [B5329F3904CC]
http://linked.open...i/riv/nazevZdroje
  • Chemistry - A European Journal
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http://linked.open...v/svazekPeriodika
  • 19
http://linked.open...iv/tvurceVysledku
  • Balintová, Jana
  • Fojta, Miroslav
  • Havran, Luděk
  • Hocek, Michal
  • Pohl, Radek
  • Vidláková, Pavlína
  • Plucnara, Medard
http://linked.open...ain/vavai/riv/wos
  • 000324316300025
issn
  • 0947-6539
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/chem.201301868
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  • 11310
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