About: The Cre/loxP recombination system for production of infectious mouse polyomavirus

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0168170213001998
Description	Murine polyomavirus mutants are frequently produced for experimental as well as therapy purposes. Commonly used methods for preparation of mutant viral genomes from recombinant vectors are laborious and give variable yields and quality. We describe an efficient and reproducible Cre/loxP-mediated recombination system that generates polyomavirus genomes from recombinant plasmid in vivo. We designed and constructed two variants of recombinant vectors containing the wild-type polyomavirus genome flanked by loxP homologous sites. The loxP sites were introduced either into the intronic region of early genes or between the two poly(A) signal sites of convergent transcriptional units. After cotransfection of the recombinant plasmids with the Cre-expressing vector into mouse 3T6 cells, we obtained infectious virus from the genome variant containing loxP site in the intronic region, but we failed to isolate any infectious virus from the viral genome containing loxP site between poly(A) signals. We show that the Cre/loxP-based method of polyomavirus production is simple, expedient, and reproducible and works with satisfactory efficiency. Murine polyomavirus mutants are frequently produced for experimental as well as therapy purposes. Commonly used methods for preparation of mutant viral genomes from recombinant vectors are laborious and give variable yields and quality. We describe an efficient and reproducible Cre/loxP-mediated recombination system that generates polyomavirus genomes from recombinant plasmid in vivo. We designed and constructed two variants of recombinant vectors containing the wild-type polyomavirus genome flanked by loxP homologous sites. The loxP sites were introduced either into the intronic region of early genes or between the two poly(A) signal sites of convergent transcriptional units. After cotransfection of the recombinant plasmids with the Cre-expressing vector into mouse 3T6 cells, we obtained infectious virus from the genome variant containing loxP site in the intronic region, but we failed to isolate any infectious virus from the viral genome containing loxP site between poly(A) signals. We show that the Cre/loxP-based method of polyomavirus production is simple, expedient, and reproducible and works with satisfactory efficiency. (en)
Title	The Cre/loxP recombination system for production of infectious mouse polyomavirus The Cre/loxP recombination system for production of infectious mouse polyomavirus (en)
skos:prefLabel	The Cre/loxP recombination system for production of infectious mouse polyomavirus The Cre/loxP recombination system for production of infectious mouse polyomavirus (en)
skos:notation	RIV/00216208:11310/13:10139602!RIV14-GA0-11310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	I P S Z
http://linked.open...avai/riv/aktivity	I, P(GPP302/10/P118), S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Suchanová, Jiřina Španielová, Hana Hron, Tomáš Forstová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	67286
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/13:10139602
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Viral genome modification; Virus production; loxP; Cre recombination; MPyV; Murine polyomavirus (en)
http://linked.open.../riv/klicoveSlovo	Cre recombination MPyV Murine polyomavirus Viral genome modification Virus production loxP
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[58188D9EC961]
http://linked.open...i/riv/nazevZdroje	Virus Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Identification of cis-acting signal and trans-acting proteins involved in DNA encapsidation and viral assembly of mouse polyomavirus.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	176
http://linked.open...iv/tvurceVysledku	Hron, Tomáš Forstová, Jitka Suchanová, Jiřina Španielová, Hana
http://linked.open...ain/vavai/riv/wos	000323858500015
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0168-1702
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.virusres.2013.05.016
http://localhost/t...ganizacniJednotka	11310

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