About: Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://abbs.oxfordjournals.org/
Description	Heterotrimeric G-proteins localized in the plasma membrane convey the signals from G-protein-coupled receptors (GPCRs) to different effectors. At least some types of G-protein α subunits have been shown to be partly released from plasma membranes and to move into the cytosol after receptor activation by the agonists. However, the mechanism underlying subcellular redistribution of trimeric G-proteins is not well understood and no definitive conclusions have been reached regarding the translocation of Gα subunits between membranes and cytosol. Here we used subcellular fractionation and clear-native polyacrylamide gel electrophoresis to identify molecular complexes of G(q/11)α protein and to determine their localization in isolated fractions and stability in naïve and thyrotropin-releasing hormone (TRH)-treated HEK293 cells expressing high levels of TRH receptor and G(11)α protein. We identified two high-molecular-weight complexes of 300 and 140 kDa in size comprising the G(q/11) protein, which were found to be membrane-bound. Both of these complexes dissociated after prolonged treatment with TRH. Still other G(q/11)α protein complexes of lower molecular weight were determined in the cytosol. These 70 kDa protein complexes were barely detectable under control conditions but their levels markedly increased after prolonged (4-16 h) hormone treatment. These results support the notion that a portion of G(q/11)α can undergo translocation from the membrane fraction into soluble fraction after a long-term activation of TRH receptor. At the same time, these findings indicate that the redistribution of G(q/11)α is brought about by the dissociation of high-molecular-weight complexes and concomitant formation of low-molecular-weight complexes containing the G(q/11)α protein. Heterotrimeric G-proteins localized in the plasma membrane convey the signals from G-protein-coupled receptors (GPCRs) to different effectors. At least some types of G-protein α subunits have been shown to be partly released from plasma membranes and to move into the cytosol after receptor activation by the agonists. However, the mechanism underlying subcellular redistribution of trimeric G-proteins is not well understood and no definitive conclusions have been reached regarding the translocation of Gα subunits between membranes and cytosol. Here we used subcellular fractionation and clear-native polyacrylamide gel electrophoresis to identify molecular complexes of G(q/11)α protein and to determine their localization in isolated fractions and stability in naïve and thyrotropin-releasing hormone (TRH)-treated HEK293 cells expressing high levels of TRH receptor and G(11)α protein. We identified two high-molecular-weight complexes of 300 and 140 kDa in size comprising the G(q/11) protein, which were found to be membrane-bound. Both of these complexes dissociated after prolonged treatment with TRH. Still other G(q/11)α protein complexes of lower molecular weight were determined in the cytosol. These 70 kDa protein complexes were barely detectable under control conditions but their levels markedly increased after prolonged (4-16 h) hormone treatment. These results support the notion that a portion of G(q/11)α can undergo translocation from the membrane fraction into soluble fraction after a long-term activation of TRH receptor. At the same time, these findings indicate that the redistribution of G(q/11)α is brought about by the dissociation of high-molecular-weight complexes and concomitant formation of low-molecular-weight complexes containing the G(q/11)α protein. (en)
Title	Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells (en)
skos:prefLabel	Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells (en)
skos:notation	RIV/00216208:11310/12:10123868!RIV13-MSM-11310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Novotný, Jiří Drastichová, Zdeňka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	140274
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10123868
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	subcellular localization; protein complex; clear native electrophoresis; trimeric Gq/11 protein (en)
http://linked.open.../riv/klicoveSlovo	clear native electrophoresis protein complex subcellular localization trimeric Gq/11 protein
http://linked.open...odStatuVydavatele	CN - Čínská lidová republika
http://linked.open...ontrolniKodProRIV	[BC5DA7757977]
http://linked.open...i/riv/nazevZdroje	Acta Biochimica et Biophysica Sinica
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	44
http://linked.open...iv/tvurceVysledku	Novotný, Jiří Drastichová, Zdeňka
http://linked.open...ain/vavai/riv/wos	000306922800003
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	1672-9145
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1093/abbs/gms050
http://localhost/t...ganizacniJednotka	11310
is http://linked.open...avai/riv/vysledek of	Identification and subcellular localization of molecular complexes of Gq/11α protein in HEK293 cells

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