About: Secondary structure is required for 3' splice site recognition in yeast.

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About: Secondary structure is required for 3' splice site recognition in yeast. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3239191/?tool=pubmed
Description	Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3' splice site (3'ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure prediction and in-line probing showed that this mutation disrupted a stem in the BP-3'ss region. Analyses of various COF1 intron modifications revealed that the secondary structure brought about the reduction of BP to 3'ss distance and masked potential 3'ss. We demonstrated the same structural requisite for the splicing of UBC13 intron. Moreover, RNAfold predicted stable structures for almost all distant BP introns in S. cerevisiae and for selected examples in several other Saccharomycotina species. The employment of intramolecular structure to localize 3'ss for the second splicing step suggests the existence of pre-mRNA structure-based mechanism of 3'ss recognition. Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3' splice site (3'ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure prediction and in-line probing showed that this mutation disrupted a stem in the BP-3'ss region. Analyses of various COF1 intron modifications revealed that the secondary structure brought about the reduction of BP to 3'ss distance and masked potential 3'ss. We demonstrated the same structural requisite for the splicing of UBC13 intron. Moreover, RNAfold predicted stable structures for almost all distant BP introns in S. cerevisiae and for selected examples in several other Saccharomycotina species. The employment of intramolecular structure to localize 3'ss for the second splicing step suggests the existence of pre-mRNA structure-based mechanism of 3'ss recognition. (en)
Title	Secondary structure is required for 3' splice site recognition in yeast. Secondary structure is required for 3' splice site recognition in yeast. (en)
skos:prefLabel	Secondary structure is required for 3' splice site recognition in yeast. Secondary structure is required for 3' splice site recognition in yeast. (en)
skos:notation	RIV/00216208:11310/11:10104338!RIV12-MSM-11310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC07032), S, Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Folk, Petr Půta, František Gahura, Ondřej Valentová, Anna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	228516
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/11:10104338
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	distal branch point; splice site recognition; UBC13; COF1; 3' splice site; intron secondary structure (en)
http://linked.open.../riv/klicoveSlovo	3' splice site COF1 UBC13 distal branch point intron secondary structure splice site recognition
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[F94803B30D0D]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Functional Genetics
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	39
http://linked.open...iv/tvurceVysledku	Folk, Petr Půta, František Gahura, Ondřej Hammann, Christian Valentová, Anna
http://linked.open...ain/vavai/riv/wos	000298186000031
http://linked.open...n/vavai/riv/zamer	Signaling and cellular response mechanisms
issn	0305-1048
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1093/nar/gkr662
http://localhost/t...ganizacniJednotka	11310
is http://linked.open...avai/riv/vysledek of	Secondary structure is required for 3' splice site recognition in yeast. Secondary structure is required for 3' splice site recognition in yeast.

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