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| Description
| - A simple and fast high-performance liquid chromatography (HPLC) method with rapid separation on monolithic column has been developed for quantitative determination of vitamin E acetate in dietary supplements. Commercial samples of dietary supplements were extracted with 100 % methanol with the help of ultrasound bath. A 20-mu L sample volume of the filtered solution was directly injected into the HPLC system. Separation of ballast matrix, vitamin E acetate, and internal standard cholecalciferol was performed on the monolithic column Chromolith Performance RP-18e column (100 x 4.6 mm) with mobile phase methanol/water (98:2, v/v) at a flow rate of 2.0 mL min(-1) and at temperature 30 A degrees C. The detector was set at 290 nm. Under the optimum chromatographic conditions, standard calibration curve was measured with good linearity-correlation coefficient for vitamin E acetate (r = 0.9992; n = 6) between the peak areas and concentrations of vitamin E acetate. Accuracy of the method defined as a mean recovery was in the range 96.4-103.6 % for all dietary supplements. The intraday method precision was satisfactory and relative standard deviation of sample analysis including preparation and determination of different dietary supplements was in the range 1.1-3.6 %. The developed method has shown high sample throughput during sample preparation process and short time (3.5 min) of analysis.
- A simple and fast high-performance liquid chromatography (HPLC) method with rapid separation on monolithic column has been developed for quantitative determination of vitamin E acetate in dietary supplements. Commercial samples of dietary supplements were extracted with 100 % methanol with the help of ultrasound bath. A 20-mu L sample volume of the filtered solution was directly injected into the HPLC system. Separation of ballast matrix, vitamin E acetate, and internal standard cholecalciferol was performed on the monolithic column Chromolith Performance RP-18e column (100 x 4.6 mm) with mobile phase methanol/water (98:2, v/v) at a flow rate of 2.0 mL min(-1) and at temperature 30 A degrees C. The detector was set at 290 nm. Under the optimum chromatographic conditions, standard calibration curve was measured with good linearity-correlation coefficient for vitamin E acetate (r = 0.9992; n = 6) between the peak areas and concentrations of vitamin E acetate. Accuracy of the method defined as a mean recovery was in the range 96.4-103.6 % for all dietary supplements. The intraday method precision was satisfactory and relative standard deviation of sample analysis including preparation and determination of different dietary supplements was in the range 1.1-3.6 %. The developed method has shown high sample throughput during sample preparation process and short time (3.5 min) of analysis. (en)
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| Title
| - A Fast HPLC Method for Determination of Vitamin E Acetate in Dietary Supplements Using Monolithic Column
- A Fast HPLC Method for Determination of Vitamin E Acetate in Dietary Supplements Using Monolithic Column (en)
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| skos:prefLabel
| - A Fast HPLC Method for Determination of Vitamin E Acetate in Dietary Supplements Using Monolithic Column
- A Fast HPLC Method for Determination of Vitamin E Acetate in Dietary Supplements Using Monolithic Column (en)
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| skos:notation
| - RIV/00216208:11160/13:10146035!RIV14-MSM-11160___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11160/13:10146035
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Dietary supplements; HPLC; Monolithic columns; Vitamin E acetate (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Solich, Petr
- Šatínský, Dalibor
- Brabcová, Ivana
- Kovářová, Lenka
- Havlíková, Lucie
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1007/s12161-012-9452-0
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| http://localhost/t...ganizacniJednotka
| |
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