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| Description
| - This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of this work was to extend the chromatographic capabilities of the SIC system. Despite the particle-packed columns reaching system pressures of {= 610 PSI, their conditions matched those of a commercially produced and optimised SIC system. The selectivity of each of the tested columns, Ascentis (R) Express RP-Amide, Ascentis (R) Express Phenyl-Hexyl and Ascentis (R) Express C18 (30 mm x 4.6 mm, core-shell particle size 2.7 mu m), was compared by their ability to separate seven phenolic acids that are secondary metabolite substances widely distributed in plants. The separations of all of the components were performed by isocratic elution using binary mobile phases composed of acetonitrile and 0.065% phosphoric acid at pH 2.4 (a specific ratio was used for each column) at a flow-rate of 0.60 mL/min. The volume of the mobile phase was 3.8 mL for each separation. The injection volume of the sample was 10 mu L for each separation. The UV detection wavelengths were set to 250, 280 and 325 nm. The RP-Amide column provided the highest chromatographic resolution and allowed for complete baseline separation of protocatechuic, syringic, vanillic, ferulic, sinapinic, p-coumaric and o-coumaric acids. The analytical parameters were a LOD of 0.3 mg L-1, a LOQof 1.0 mg L-1, a calibration range of 1.0-50.0 (100.0) mg L-1 (r > 0.997) and a system precision of 10 mg L-1 with a RSD {= 1.65%. The high performance of the chromatography process with the RP-Amide column under optimised conditions was highlighted and well documented (HETP values {= 10 mu m, peak symmetry {= 1.33, resolution }= 1.87 and time for one analysis <8.0 min). The results of these experiments confirmed the benefits of extending chromatographic selectivity using core-shell particle column technology in a SIC manifold.
- This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of this work was to extend the chromatographic capabilities of the SIC system. Despite the particle-packed columns reaching system pressures of {= 610 PSI, their conditions matched those of a commercially produced and optimised SIC system. The selectivity of each of the tested columns, Ascentis (R) Express RP-Amide, Ascentis (R) Express Phenyl-Hexyl and Ascentis (R) Express C18 (30 mm x 4.6 mm, core-shell particle size 2.7 mu m), was compared by their ability to separate seven phenolic acids that are secondary metabolite substances widely distributed in plants. The separations of all of the components were performed by isocratic elution using binary mobile phases composed of acetonitrile and 0.065% phosphoric acid at pH 2.4 (a specific ratio was used for each column) at a flow-rate of 0.60 mL/min. The volume of the mobile phase was 3.8 mL for each separation. The injection volume of the sample was 10 mu L for each separation. The UV detection wavelengths were set to 250, 280 and 325 nm. The RP-Amide column provided the highest chromatographic resolution and allowed for complete baseline separation of protocatechuic, syringic, vanillic, ferulic, sinapinic, p-coumaric and o-coumaric acids. The analytical parameters were a LOD of 0.3 mg L-1, a LOQof 1.0 mg L-1, a calibration range of 1.0-50.0 (100.0) mg L-1 (r > 0.997) and a system precision of 10 mg L-1 with a RSD {= 1.65%. The high performance of the chromatography process with the RP-Amide column under optimised conditions was highlighted and well documented (HETP values {= 10 mu m, peak symmetry {= 1.33, resolution }= 1.87 and time for one analysis <8.0 min). The results of these experiments confirmed the benefits of extending chromatographic selectivity using core-shell particle column technology in a SIC manifold. (en)
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| Title
| - Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids
- Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids (en)
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| skos:prefLabel
| - Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids
- Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids (en)
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| skos:notation
| - RIV/00216208:11160/13:10145719!RIV14-MSM-11160___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11160/13:10145719
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Phenolic acids; Columns comparison; Phenyl-Hexyl; RP-C18; RP-Amide; Core-shell particle column; Sequential Injection Chromatography (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Solich, Petr
- Chocholouš, Petr
- Šatínský, Dalibor
- Šrámková, Ivana
- Vacková, Jana
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
| - 10.1016/j.talanta.2012.10.036
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| http://localhost/t...ganizacniJednotka
| |
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