About: Efficient isolation of carbonyl-reducing enzymes using affinity approach with anticancer drug oracin as a specific ligand

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About: Efficient isolation of carbonyl-reducing enzymes using affinity approach with anticancer drug oracin as a specific ligand Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/jssc.201201141/full
Description	Carbonyl-reducing enzymes are important in both metabolism of endogenous substances and biotransformation of xenobiotics. Because sufficient amounts of native enzymes must be obtained to study their roles in metabolism, an efficient purification strategy is very important. Oracin (6-[2-(2-hydroxyethyl)aminoethyl]-5,11-dioxo-5,6-dihydro-11H-indeno[1,2-c]isoquinoline) is a prospective anticancer drug and one of the xenobiotic substrates for carbonyl-reducing enzymes. A new purification strategy based on molecular recognition of carbonyl-reducing enzymes with oracin as a ligand is reported here. The type of covalent bond, ligand molecules orientation, and their distance from the backbone of the solid matrix for good stearic accessibility were taken into account during the designing of the carrier. The carriers based on magnetically active microparticles were tested by recombinant enzymes AKR1C3 and CBR1. The SiMAG-COOH magnetic microparticles with N-alkylated oracin and BAPA as spacer arm provide required parameters: proper selectivity and specificity enabling to isolate the target enzyme in sufficient quantity, purity, and activity. Carbonyl-reducing enzymes are important in both metabolism of endogenous substances and biotransformation of xenobiotics. Because sufficient amounts of native enzymes must be obtained to study their roles in metabolism, an efficient purification strategy is very important. Oracin (6-[2-(2-hydroxyethyl)aminoethyl]-5,11-dioxo-5,6-dihydro-11H-indeno[1,2-c]isoquinoline) is a prospective anticancer drug and one of the xenobiotic substrates for carbonyl-reducing enzymes. A new purification strategy based on molecular recognition of carbonyl-reducing enzymes with oracin as a ligand is reported here. The type of covalent bond, ligand molecules orientation, and their distance from the backbone of the solid matrix for good stearic accessibility were taken into account during the designing of the carrier. The carriers based on magnetically active microparticles were tested by recombinant enzymes AKR1C3 and CBR1. The SiMAG-COOH magnetic microparticles with N-alkylated oracin and BAPA as spacer arm provide required parameters: proper selectivity and specificity enabling to isolate the target enzyme in sufficient quantity, purity, and activity. (en)
Title	Efficient isolation of carbonyl-reducing enzymes using affinity approach with anticancer drug oracin as a specific ligand Efficient isolation of carbonyl-reducing enzymes using affinity approach with anticancer drug oracin as a specific ligand (en)
skos:prefLabel	Efficient isolation of carbonyl-reducing enzymes using affinity approach with anticancer drug oracin as a specific ligand Efficient isolation of carbonyl-reducing enzymes using affinity approach with anticancer drug oracin as a specific ligand (en)
skos:notation	RIV/00216208:11160/13:10144066!RIV14-MSM-11160___
http://linked.open...avai/predkladatel	Farmaceutická fakulta v Hradci Králové
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(EE2.3.20.0235), P(GAP206/12/0381), S
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Štambergová, Hana Wsól, Vladimír Škarydová, Lucie Andrýs, Rudolf
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Farmaceutická fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	72078
http://linked.open...ai/riv/idVysledku	RIV/00216208:11160/13:10144066
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Reductase; Oracin; Magnetic; Carbonyl; Affinity (en)
http://linked.open.../riv/klicoveSlovo	Oracin Magnetic Reductase Affinity Carbonyl
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[17A0A34CDC5F]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of ultrasensitive electrochemical immunosensors for early detection of clinically significant biomarkers of malignant diseases Establishment of Research Team Focused on Experimental and Applied Biopharmacy
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	36
http://linked.open...iv/tvurceVysledku	Wsól, Vladimír Škarydová, Lucie Štambergová, Hana Bílková, Zuzana Holubová, Lucie Andrýs, Rudolf Kňavová, Jana
http://linked.open...ain/vavai/riv/wos	000319865700003
issn	1615-9306
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jssc.201201141
http://localhost/t...ganizacniJednotka	11160

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