About: The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

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About: The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.spandidos-publications.com/or/30/6/2593
Description	In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 µM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of α-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of α-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of α-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in α-tomatine-treated cells. These results support the conclusion that α-tomatine does not induce apoptosis in the MCF-7 cell line. In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 µM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of α-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of α-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of α-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in α-tomatine-treated cells. These results support the conclusion that α-tomatine does not induce apoptosis in the MCF-7 cell line. (en)
Title	The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction (en)
skos:prefLabel	The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction (en)
skos:notation	RIV/00216208:11150/13:10173671!RIV14-GA0-11150___
http://linked.open...avai/predkladatel	Lékařská fakulta v Hradci Králové
http://linked.open...avai/riv/aktivita	I P S
http://linked.open...avai/riv/aktivity	I, P(EE2.3.30.0022), P(GPP303/12/P536), S
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hroch, Miloš Ćmielová, Jana Řezáčová, Martina Havelek, Radim Šišpera, Luděk Bezrouk, Aleš Rudolf, Emil Tomšík, Pavel Suchá, Lenka Köhlerová, Renata
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	67674
http://linked.open...ai/riv/idVysledku	RIV/00216208:11150/13:10173671
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cholesterol; cell death; MCF-7; alpha-tomatine (en)
http://linked.open.../riv/klicoveSlovo	MCF-7 alpha-tomatine cell death cholesterol
http://linked.open...odStatuVydavatele	GR - Řecká republika
http://linked.open...ontrolniKodProRIV	[53479F992835]
http://linked.open...i/riv/nazevZdroje	Oncology Reports
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	The Use of Tomatine, a Tomato Alkaloid, in the Therapy of Cancer Support of establishment, development, and mobility of quality research teams at the Charles University
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	30
http://linked.open...iv/tvurceVysledku	Havelek, Radim Rudolf, Emil Tomšík, Pavel Řezáčová, Martina Šišpera, Luděk Hroch, Miloš Ćmielová, Jana Suchá, Lenka Köhlerová, Renata Bezrouk, Aleš
http://linked.open...ain/vavai/riv/wos	000330226200007
issn	1021-335X
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.3892/or.2013.2778
http://localhost/t...ganizacniJednotka	11150

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