About: Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The MALDI-TOF MS is an ideal method for routine identification of proteins separated by two-dimensional polyacrylamide gel electrophoresis (2D-P AGE). However, in some cases the outputs from peptide mass fingerprinting (PMF) do not enable unambiguous identification of proteins. In such situation, determination of a partial amino acid sequence of the target protein would be extremely helpful in the PMF identification. Sequencing using MALDI-TOF post-source decay (PSD) usually generates complex spectra and the fragmentation is not complete. PSD sequencing with chemically assisted fragmentation allows to avoid these problems. Application of both methods in identification of proteins of Francisella tularensis is presented. The MALDI-TOF MS is an ideal method for routine identification of proteins separated by two-dimensional polyacrylamide gel electrophoresis (2D-P AGE). However, in some cases the outputs from peptide mass fingerprinting (PMF) do not enable unambiguous identification of proteins. In such situation, determination of a partial amino acid sequence of the target protein would be extremely helpful in the PMF identification. Sequencing using MALDI-TOF post-source decay (PSD) usually generates complex spectra and the fragmentation is not complete. PSD sequencing with chemically assisted fragmentation allows to avoid these problems. Application of both methods in identification of proteins of Francisella tularensis is presented. (en) MALDI-TOF MS je ideální metodou pro rutinní identifikaci bílkovin separovaných dvojrozměrnou elektroforézou na polyakrylamidovém gelu (2D-PAGE). Nicméně v některých případech výsledky z fingerprintingu peptidové hmoty (PMF) neumožní jednoznačnou identifikaci bílkovin. V takových situacích by bylo určení částečné aminokyselinové sekvence cílové bílkoviny u PMF identifikací velmi prospěšné. Sekvenace za pomoci MALDI-TOF post-source decay (PSD) obvykle vytváří komplexní spektra a fragmentace není dokončena. PSD sekvenace spolu s chemicky podporovanou fragmentací umožňuje se vyhnout těmto problémům. V následující části tohoto sdělení je prezentována aplikace obou metod při identifikaci bílkovin z Francisely tularensis. (cs)
Title	Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides Identifikace proteinů pomocí kombinace proteinové hmotností spektrometrie a fragmentace sulfonovaných proteinů (cs) Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides (en)
skos:prefLabel	Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides Identifikace proteinů pomocí kombinace proteinové hmotností spektrometrie a fragmentace sulfonovaných proteinů (cs) Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides (en)
skos:notation	RIV/00216208:11150/07:00004060!RIV08-MSM-11150___
http://linked.open.../vavai/riv/strany	264;267
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Stulík, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Lékařská fakulta v Hradci Králové
http://linked.open...dnocenehoVysledku	425310
http://linked.open...ai/riv/idVysledku	RIV/00216208:11150/07:00004060
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Identification; Proteins; Combination; Peptide; Fingerprinting; Fragmentation; Sulfonated; Peptides (en)
http://linked.open.../riv/klicoveSlovo	Sulfonated Peptide Combination Proteins Fragmentation Identification Peptides Fingerprinting
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[42985FF0EB38]
http://linked.open...i/riv/nazevZdroje	Chemické listy
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	98
http://linked.open...iv/tvurceVysledku	Stulík, Jiří
issn	0009-2770
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	11150
is http://linked.open...avai/riv/vysledek of	Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides Identification of Proteins by Combination of Peptide Mass Fingerprinting and Fragmentation of Sulfonated Peptides

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