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| Description
| - Using high-resolution oxygraphy we tested changes of various parameters characterizing the mitochondrial energy provision system that were induced by oxidative damage.In the presence of succinate as respiratory substrate,3 mM t-butyl hydroperoxide increased respiration in the absence of ADP,which indicated partial uncopling of oxidative phosphorylation.Low activity of coupled respiration was still maintained.However,during the incubation the phosphorylative capacity decreased as indicated by the continous decrease of the mitochondrial membrane potential.Under these experimental conditions the maximum capacity of the succinate oxidase system was inhibited by 50% in comparison with values obtained in the absence of t-butyl hydroperoxide.Our data thus indicate that the oxygraphic evaluation of mitochondrial function represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism.
- Using high-resolution oxygraphy we tested changes of various parameters characterizing the mitochondrial energy provision system that were induced by oxidative damage.In the presence of succinate as respiratory substrate,3 mM t-butyl hydroperoxide increased respiration in the absence of ADP,which indicated partial uncopling of oxidative phosphorylation.Low activity of coupled respiration was still maintained.However,during the incubation the phosphorylative capacity decreased as indicated by the continous decrease of the mitochondrial membrane potential.Under these experimental conditions the maximum capacity of the succinate oxidase system was inhibited by 50% in comparison with values obtained in the absence of t-butyl hydroperoxide.Our data thus indicate that the oxygraphic evaluation of mitochondrial function represents a useful tool for evaluation of changes participating in peroxidative damage of cell energy metabolism. (en)
- Pomocí vysokoúčinné oxygrafie jsme testovali efekt oxidačního poškození na různé parametry mitochondriálního energetického metabolismu. 3 mM t-butylhydroperoxid zvýšil respiraci indukovanou sukcinátem bez přítomnosti ADP. To značí částečné rozpřažení oxidační fosforylace.Během inkubace se rovněž snižoval mitochondriální membránový potenciál,což svědčí o snížené fosforylační kapacitě.Z našich experimentálních podmínek byla maximální kapacita sukcinát dehydrogenázy inhibována o 50 % ve srovnání s kontrolními hodnotami.Naše data značí, že oxygrafie je vhodná metoda pro hodnocení změn energetického metabolismu navozených oxidačním poškozením. (cs)
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| Title
| - Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes
- Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes (en)
- Inhibiční účinek t-butylhydroperoxidu na mitochondriální oxidační fosforylaci u izolovaných potkaních hepatocytů (cs)
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| skos:prefLabel
| - Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes
- Inhibitory effect of t-butyl hydroperoxide on mitochondrial oxidative phosphorylation in isolated rat hepatocytes (en)
- Inhibiční účinek t-butylhydroperoxidu na mitochondriální oxidační fosforylaci u izolovaných potkaních hepatocytů (cs)
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| skos:notation
| - RIV/00216208:11150/07:00003879!RIV08-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA303/06/1261), P(GD303/03/H065), S, Z(AV0Z50110509), Z(MSM 111100008), Z(MSM0021620820)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/07:00003879
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - hepatocytes; oxidative phoshorylation; t-butyl hydroperoxide (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Červinková, Zuzana
- Křiváková, Pavla
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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