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  • Percentage of serological positivity examined in 4205 blood sera by serological method microscopic agglutination test (MAT) on the hinterland territory of our laboratory (East Bohemia; 1999-2003) was 0.38-4.7 %. By the PCR method for detection of DNA of pathogenic leptospires (L. interrogans, L. borgpetersenii and L. kirschneri) from 57 samples of different biological materials from patients with fever of unknown etiology positive results were obtained in 4 specimens (7 %; 3 samples of urine and 1 sample of blood). This method was shown to distinguish between pathogenic and nonpathogenic strains and can detect 2.5-10 cells per mL of biological material. As an important presumption of successful detection of pathogenic leptospires a correct collecting of blood, urine samples or liquor is required before starting antibody therapy.
  • Percentage of serological positivity examined in 4205 blood sera by serological method microscopic agglutination test (MAT) on the hinterland territory of our laboratory (East Bohemia; 1999-2003) was 0.38-4.7 %. By the PCR method for detection of DNA of pathogenic leptospires (L. interrogans, L. borgpetersenii and L. kirschneri) from 57 samples of different biological materials from patients with fever of unknown etiology positive results were obtained in 4 specimens (7 %; 3 samples of urine and 1 sample of blood). This method was shown to distinguish between pathogenic and nonpathogenic strains and can detect 2.5-10 cells per mL of biological material. As an important presumption of successful detection of pathogenic leptospires a correct collecting of blood, urine samples or liquor is required before starting antibody therapy. (en)
  • Procento sérologické pozitivity vyšetřované u 4205 lidských sér pomocí mikroskopického aglutinačního testu (MAT) v teritoriální oblasti naší laboratoře (Východní Čechy; 1999-2003) bylo 0,38-4,7 %. Detekce DNA patogenních leptospir (L. interrogans, L. borgpetersenii a L. kirschneri) pomocí PCR byla provedena u 57 vzorků různých biologických materiálů od pacientů trpících horečkou neznámého původu a ve 4 vzorcích (7 %; 3 vzorky moči a 1 vzorek krve) byly získány pozitivní údaje. Tato metoda je schopná odlišit patogenní a nepatogenní kmeny a je schopná zachytit 2,5-10 buněk na 1 ml biologického materiálu. Důležitou presumpcí úspěšné detekce patogenních leptospir je nutnost správně odebírat krevní a močové vzorky a likvor před zahájením terapie protilátkami. PCR metoda je jasně výhodnější než ostatní diagnostické metody jako např. MAT, která detekuje přítomnost protilátek v krvi nakaženého člověka, které se však objeví až mnoho dní po zahájení infekce. (cs)
Title
  • Laboratory Diagnosis of Leptospirosis
  • Laboratorní diagnóza leptospirózy (cs)
  • Laboratory Diagnosis of Leptospirosis (en)
skos:prefLabel
  • Laboratory Diagnosis of Leptospirosis
  • Laboratorní diagnóza leptospirózy (cs)
  • Laboratory Diagnosis of Leptospirosis (en)
skos:notation
  • RIV/00216208:11150/05:00004932!RIV08-MSM-11150___
http://linked.open.../vavai/riv/strany
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  • 527659
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  • RIV/00216208:11150/05:00004932
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  • Laboratory; Diagnosis; Leptospirosis (en)
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http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [BBA62AD10D4B]
http://linked.open...i/riv/nazevZdroje
  • Folia Microbiologica
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 50
http://linked.open...iv/tvurceVysledku
  • Ryšková, Olga
  • Čermáková, Zuzana
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  • 0015-5632
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