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| Description
| - Toxoplazma gondii byla detekována v biologických vzorcích ze 347 jedinců (441 biologických materiálů) pomocí polymerázové řetězové reakce (PCR) genů TGR1E (primery TGR1E-1 a TGR1E-2 – PCR standard) a B1 (primery TM1, TM2, TM3). T. gondii byla nalezena v 5,2 % v následujících biologických vzorcích: krev (6), krev novorozenců (2), biopsie (2), vzorky progenitorových buněk (2; od kandidátů na transplantaci kostní dřeně). DNA z T. gondii byla rovněž nalezena v 11 vzorcích (8,3 %) ze 120 těhotných žen během prenatálního vyšetřování. Pozitivní byl i test v krevních vzorcích dvou novorozenců od matek, které se infikovaly v pozdních fázích těhotenství. Pozitivita PCR reakce byla potvrzena sérologickými testy (ELISA a komplement fixační test) a shoda PCR pozitivity a nalezených protilátek v krvi byla 83,3 %. Rychlé potvrzení akutní přítomnosti T. gondii je zejména důležité tam, kde infekce může mít závažné budoucí dopady (např. pro plod těhotných žen či u imunosuprimovaných pacientů). (cs)
- Using the polymerase chain reaction (PCR), Toxoplasma gondii from gene TGR1E with primers TGR1E-1, TGR1E-2 (standard PCR), and from B1 gene with primers TM1, TM2, TM3 (hemi-nested PCR) was detected in biological samples from 347 individuals (441 biological materials). Of the total of 441 biological materials, T. gondii DNA was detected in 5.2 %; it was positive in the following samples: blood (n = 6), blood from newborns, biopsies and samples of progenitor cells (from candidates for bone marrow transplantation). DNA of T. gondii was also revealed in 11 samples (8.3 %) of 120 cases of pregnant women during prenatal examinations. A positive result in the blood was also found in two cases of newborn babies from mothers who were infected in later pregnancy. The positive PCR examination was confirmed by serological methods (ELISA and complement fixation test). Agreement of PCR results and the detection of antibodies against toxoplasma was found in 83.3 %.
- Using the polymerase chain reaction (PCR), Toxoplasma gondii from gene TGR1E with primers TGR1E-1, TGR1E-2 (standard PCR), and from B1 gene with primers TM1, TM2, TM3 (hemi-nested PCR) was detected in biological samples from 347 individuals (441 biological materials). Of the total of 441 biological materials, T. gondii DNA was detected in 5.2 %; it was positive in the following samples: blood (n = 6), blood from newborns, biopsies and samples of progenitor cells (from candidates for bone marrow transplantation). DNA of T. gondii was also revealed in 11 samples (8.3 %) of 120 cases of pregnant women during prenatal examinations. A positive result in the blood was also found in two cases of newborn babies from mothers who were infected in later pregnancy. The positive PCR examination was confirmed by serological methods (ELISA and complement fixation test). Agreement of PCR results and the detection of antibodies against toxoplasma was found in 83.3 %. (en)
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| Title
| - Polymerase Chain Reaction for detection of Toxoplasma gondii in Human Biological Samples
- PCR pro detekci Toxoplasma gondii v lidských biologických vzorcích (cs)
- Polymerase Chain Reaction for detection of Toxoplasma gondii in Human Biological Samples (en)
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| skos:prefLabel
| - Polymerase Chain Reaction for detection of Toxoplasma gondii in Human Biological Samples
- PCR pro detekci Toxoplasma gondii v lidských biologických vzorcích (cs)
- Polymerase Chain Reaction for detection of Toxoplasma gondii in Human Biological Samples (en)
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| skos:notation
| - RIV/00216208:11150/05:00004928!RIV08-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/05:00004928
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Polymerase; Chain; Reaction; detection; Toxoplasma; gondii; Human; Biological; Samples (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Ryšková, Olga
- Čermáková, Zuzana
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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