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| Description
| - Antiborrelia antibodies in Lyme borreliosis (LB) are mostly detected by enzyme immunoassay (EIA), confirmed by immunoblot (the %22two-step system%22). In indicated cases, direct evidence of Borrelia burgdorferi is obtained with the PCR method, electron microscopy and cultivation. The %22one-step system%22 of testing for IgM and IgG antibodies in LB is economically preferably, but it requires an EIA kit with more than 90% sensitivity and specificity. The results were verified clinically in all cases, in disputable cases with additional immunoblot (BAG-Med), and analyzed statistically. Conclusions: The best results for a %22one-step system%22 of examinations for antiborrelia antibodies were obtained with the Abbot and Euroimmun EIA kits in our set. A %22two-step system%22 of serological examination could be composed from the basic IgM and IgG examination with a high sensitivity EIA kit (Viroimmun, Test-Line) followed with confirmation of positives by specific immunoblot.
- Antiborrelia antibodies in Lyme borreliosis (LB) are mostly detected by enzyme immunoassay (EIA), confirmed by immunoblot (the %22two-step system%22). In indicated cases, direct evidence of Borrelia burgdorferi is obtained with the PCR method, electron microscopy and cultivation. The %22one-step system%22 of testing for IgM and IgG antibodies in LB is economically preferably, but it requires an EIA kit with more than 90% sensitivity and specificity. The results were verified clinically in all cases, in disputable cases with additional immunoblot (BAG-Med), and analyzed statistically. Conclusions: The best results for a %22one-step system%22 of examinations for antiborrelia antibodies were obtained with the Abbot and Euroimmun EIA kits in our set. A %22two-step system%22 of serological examination could be composed from the basic IgM and IgG examination with a high sensitivity EIA kit (Viroimmun, Test-Line) followed with confirmation of positives by specific immunoblot. (en)
- Úvod: protilátky proti borélii u Lymeské boreliózy (LB) se většinou detekují dvoustupňovým procesem (EIA + blot). V daných případech je přímý důkaz přítomnosti borélie poskytnut PCR, elektronovou mikroskopií nebo kultivačně. Jednostupňový proces detekc protilátek IgM a IgG u LB je ekonomický, ale vyžaduje vysokou specifitu a citlivost (více než 90%) EIA kitu. Materiál a metody: bylo shromážděno 90 krevních vzorků (54 od pacientů s LB a 36 ze zdravých jedinců). Protilátky IgM a IgG byly detekovány 5 EIA kity od různých výrobců. Výsledky byly verifikovány klinicky, v případě nejasností i pomocí imunoblotu (BAG-med). Závěry: nejlepší výsledky s jednostupňovou detekcí byly získány s EIA kity od Abbot a Eurimmun. Dvoustupňová sérologická detekce by se mohla skládat ze základní detekce IgM a IgG plus vysoce citlivých EIA kitů (Viroimmun, Test-Line) s následným potvrzením pozitivity spedifickým imunoblotem. (cs)
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| Title
| - Diagnosis of Lyme borreliosis using enzyme immunoanalysis
- Diagnostika Lymské borreliózy při použití EIA metod (cs)
- Diagnosis of Lyme borreliosis using enzyme immunoanalysis (en)
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| skos:prefLabel
| - Diagnosis of Lyme borreliosis using enzyme immunoanalysis
- Diagnostika Lymské borreliózy při použití EIA metod (cs)
- Diagnosis of Lyme borreliosis using enzyme immunoanalysis (en)
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| skos:notation
| - RIV/00216208:11150/05:00004048!RIV08-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/05:00004048
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Diagnosis; borreliosis; using; enzyme; immunoanalysis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Čermáková, Eva
- Hanovcová, Irena
- Ryšková, Olga
- Čermáková, Zuzana
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| issn
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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