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| Description
| - Zinc is an important cellular antioxidant. We investigated its role in chromium-induced oxidative stress and apoptosis in human tumor cell line Hep-2. The measured parameters included intracellular labile zinc content (Zinquin-E fluorescence), cell viability (WST-1 assay), oxidative stress (spectrophotometry), mitochondrial potential (flow cytometry), caspase-3 activity, and PARP cleavage (immunofluoroscence). We found that Hep-2 cells contain abundant labile zinc stores that may be depleted by the ionophore TPEN or increased by external zinc supplementation. Chromium (VI)-induced cytotoxicity and apoptosis were enhanced in zinc-depleted cells after 24 h, in particular at chromium (VI) concentrations of 50 umol/l. On the other hand, elevated levels of labile zinc were able to protect against apoptosis induced by 10 umol/l chromium (VI) but at higher chromium (VI) concentrations (50 and 150 umol/l) acted synergistically, significantly enhancing oxidative stress and the course of apoptosis.
- Zinc is an important cellular antioxidant. We investigated its role in chromium-induced oxidative stress and apoptosis in human tumor cell line Hep-2. The measured parameters included intracellular labile zinc content (Zinquin-E fluorescence), cell viability (WST-1 assay), oxidative stress (spectrophotometry), mitochondrial potential (flow cytometry), caspase-3 activity, and PARP cleavage (immunofluoroscence). We found that Hep-2 cells contain abundant labile zinc stores that may be depleted by the ionophore TPEN or increased by external zinc supplementation. Chromium (VI)-induced cytotoxicity and apoptosis were enhanced in zinc-depleted cells after 24 h, in particular at chromium (VI) concentrations of 50 umol/l. On the other hand, elevated levels of labile zinc were able to protect against apoptosis induced by 10 umol/l chromium (VI) but at higher chromium (VI) concentrations (50 and 150 umol/l) acted synergistically, significantly enhancing oxidative stress and the course of apoptosis. (en)
- Zkoumali jsme vliv zinku na chromem vyvolaný oxidační stres a apoptózu u lidské nádorové linie HEP-2. Zkoumané parametry zahrnovaly: intracelulární koncentrace zinku (fluorescence Zinquin-E), buněčná viabilita (WST-1), oxidační stres (spektrofotometrie), mitochondrální potenciál (průtoková cytometrie), aktivita kaspázy 3 a štěpení PARP (imunofluorescence). Zjistili jsme, že buňky HEP-2 obsahují poměrně vysoké zásoby intracelulárního zinku, které mohou být sníženy aplikací ionoforu TPEN a nebo zvýšeny suplementací. Chromem vyvolaná cytotoxicita a apoptóza u těchto buněk byla zesílená v přítomnosti nižších intracelulárních koncentracích zinku (24 hodin), a to zejména při koncentracích chromu 50 a 150 mikromol. Na druhé straně zvýšené koncentrace intracelulárního zinku dokázaly ochránit buňku před toxickými účinky chromu (10 mikromol). V případě vyšších koncentrací chromu však zinek toxické účinky potencoval prostřednictvím oxidačního stresu a mitochondriálního poškození. (cs)
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| Title
| - Zinc has ambiguous effects on chromium (VI)-induced oxidative stress and apoptosis
- Zinc has ambiguous effects on chromium (VI)-induced oxidative stress and apoptosis (en)
- Ambivalentní účinky zinku na chromem (VI) vyvolaný oxidační stres a apoptózu (cs)
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| skos:prefLabel
| - Zinc has ambiguous effects on chromium (VI)-induced oxidative stress and apoptosis
- Zinc has ambiguous effects on chromium (VI)-induced oxidative stress and apoptosis (en)
- Ambivalentní účinky zinku na chromem (VI) vyvolaný oxidační stres a apoptózu (cs)
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| skos:notation
| - RIV/00216208:11150/05:00003634!RIV06-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - Z(MSM 111500001), Z(MSM 111500002)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/05:00003634
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Zinc; hexavalent chromium; apoptosis; oxidative stress; mitochondria (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Trace Elements in Medicine and Biology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Cerman, Jaroslav
- Rudolf, Emil
- Červinka, Miroslav
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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