About: Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle     Goto   Sponge   NotDistinct   Permalink

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  • We investigated direct effects of peptide vinylsulfone inhibitor AdaAhx3L3VS on protein and amino acids metabolism in rat skeletal muscle.M.soleus and m.extensor digitorum longus were incubated in metidum containing 30 umol/l AdaAhx3L3VS or no inhibitors.Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation.The rates of leucine oxidation and protein synthesis were evaluated during incubation in medium containing L-[1-14C]leucine.Amino acid concentrations in the medium were measured using HPLC.Statistical comparisons were performed by paired t-tests.AdaAhx3L3VS decreased both proteolysis (by 20%) and protein synthesis (by 10-20%)and increased leucine oxidation.The release of a number of amino acids into the medium was lowered.We conclude that AdaAhx3L3VS decreased overall protein turnover in the muscle tissue.
  • We investigated direct effects of peptide vinylsulfone inhibitor AdaAhx3L3VS on protein and amino acids metabolism in rat skeletal muscle.M.soleus and m.extensor digitorum longus were incubated in metidum containing 30 umol/l AdaAhx3L3VS or no inhibitors.Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation.The rates of leucine oxidation and protein synthesis were evaluated during incubation in medium containing L-[1-14C]leucine.Amino acid concentrations in the medium were measured using HPLC.Statistical comparisons were performed by paired t-tests.AdaAhx3L3VS decreased both proteolysis (by 20%) and protein synthesis (by 10-20%)and increased leucine oxidation.The release of a number of amino acids into the medium was lowered.We conclude that AdaAhx3L3VS decreased overall protein turnover in the muscle tissue. (en)
  • V této práci jsme studovali účinek inhibitoru AdaAhx3L3VS na metabolismus proteinů a aminokyselin v kosterním svalu potkana.M.soleus a m.extensor digitorum longus byly inkubovány v médiu obsahujícím 30 uml/l AdaAhx3L3VS nebo bez inhibitor.Proteolýza byla hodnocena na základě množství tyrosinu uvolněného do inkubačního média.Oxidace leucinu a proteosyntéza byly hodnoceny pomocí L-[1-14C] leucinu.Koncentrace aminokyselin v médiu byly měřeny pomocí HPLC.Statistické hodnocení bylo provedeno párovým t-testem.Přidání AdaAhx3L3VS do inkubačního média snížilo proteolýzu o 20 % a proteosyntézu o 10-20 % a zvýšilo oxidaci leucinu.Do média se snížilo uvolňování řady aminokyselin.Uvedené výsledky ukazují,že pridání AdaAhx3L3VS k médiu snižuje obrat proteinů v inkubovaném kosterním svalu. (cs)
Title
  • Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle
  • Přímý účinek inhibitoru proteasomu AdaAhx3L3VS na metabolismus proteinů a aminokyselin (cs)
  • Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle (en)
skos:prefLabel
  • Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle
  • Přímý účinek inhibitoru proteasomu AdaAhx3L3VS na metabolismus proteinů a aminokyselin (cs)
  • Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle (en)
skos:notation
  • RIV/00216208:11150/05:00003533!RIV06-MSM-11150___
http://linked.open.../vavai/riv/strany
  • 541;547
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA303/03/1512), Z(MSM0021620820)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 518203
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  • RIV/00216208:11150/05:00003533
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http://linked.open.../riv/klicovaSlova
  • proteasome inhibitor; peptide vinylsulfone; protein metaoblism amino acids (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [AFC5DD328936]
http://linked.open...i/riv/nazevZdroje
  • Physiological Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 54
http://linked.open...iv/tvurceVysledku
  • Holeček, Milan
  • Šafránek, Roman
http://linked.open...n/vavai/riv/zamer
issn
  • 0862-8408
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http://localhost/t...ganizacniJednotka
  • 11150
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