| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - V práci je posouzen účinek inhibitorů proteasomů-peptidového aldehydu MG132,2 peptidických vinylsulfonů ZL3VS a AdaAhx3L3VS na metabolismus proteinů u sepse. Sepse byla indukována ligací a následnou punkcí slepého střeva. Parametry metabolismu proteinů byly stanoveny na modelu inkubovaného kosterního svalu. Proteolýza byla vypočtena na základě uvolnění tyrosinu do inkubačního média. Proteosyntéza a oxidace leucinu pomocí L-/1-14C/leucinu. Inhibitory proteasomů neměly vliv na proteosyntézu. MG132 snížil proteolýzu o více než 50 %, AdaAhx3L3VS o 20 % a účinek ZL3VS byl nesignifikantní. Závěrem je konstatováno, že MG132 a AdaAHx3L3VS eliminovaly proteokatabolismus indukovaný sepsí. Mechanismem bylo snížení proteolýzy a ne stimulace proteosyntézy. (cs)
- We evaluated the effects of proteasome inhibitors - a peptide aldehyde MG132, and two vinylsulfone inhibitors ZL3VS and AdaAhx3L3VS - on protein metabolism in sepsis. Sepsis was induced in rats by caecal ligation and puncture. Parameters of protein metabolism were measured in incubated rat skeletal muscles. Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation. The ratesof protein synthesis and leucine oxidation were evaluated by incubating muslces in medium containing L-/1-14C/leucine. In the septic muscles, all three inhibitors testedhad no effect on protein synthesis.MG132 decreased proteolysis by more then 50%, AdaAhx3L3VS by 20%, while the effect of ZL3VS was not significant. We conclude, that MG132 and AdaAhx3L3VS reversed at least partly the protein catabolism in sepsis, as they decreased the proteolysis and did not change the protein synthesis.
- We evaluated the effects of proteasome inhibitors - a peptide aldehyde MG132, and two vinylsulfone inhibitors ZL3VS and AdaAhx3L3VS - on protein metabolism in sepsis. Sepsis was induced in rats by caecal ligation and puncture. Parameters of protein metabolism were measured in incubated rat skeletal muscles. Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation. The ratesof protein synthesis and leucine oxidation were evaluated by incubating muslces in medium containing L-/1-14C/leucine. In the septic muscles, all three inhibitors testedhad no effect on protein synthesis.MG132 decreased proteolysis by more then 50%, AdaAhx3L3VS by 20%, while the effect of ZL3VS was not significant. We conclude, that MG132 and AdaAhx3L3VS reversed at least partly the protein catabolism in sepsis, as they decreased the proteolysis and did not change the protein synthesis. (en)
|
| Title
| - Vliv inhibitorů proteasomu MG132, ZL3VS a AdaAhX3-L3VS na metabolismus proteinů u potkanů v sepsi (cs)
- Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats
- Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats (en)
|
| skos:prefLabel
| - Vliv inhibitorů proteasomu MG132, ZL3VS a AdaAhX3-L3VS na metabolismus proteinů u potkanů v sepsi (cs)
- Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats
- Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats (en)
|
| skos:notation
| - RIV/00216208:11150/04:00002083!RIV/2005/GA0/111505/N
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/04:00002083
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - proteasome inhibitors;protein metabolism;sepsis (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - International Journal of Experimental Pathology
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Holeček, Milan
- Šafránek, Roman
|
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)