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| Description
| - This work describes the biotransformation of F enantiomers and DHF stereoisomers in isolated male guinea pig hepatocytes. Guinea pigs were chosen with respect to similarities in F metabolism as in Man found earlier. R-F, S-F, (2R;4S)-DHF, (2S;4R)-DHF, (2S;4S)-DHF and (2R;4R)-DHF, structurally very similar compounds, served as substrates in order to observe their interaction with enzymes. Stereospecificity of the respective enzymes was studied in vitro, using hepatocytes monolayer. Chiral HPLC using R,R-ULMO column as chiral stationary phase was used for detection and quantitation of metabolites. Conclusions: Different activities and stereospecificities of the respective enzymes were observed for each substrate in primary culture of hepatocytes. Cell integrity is crucial for formation of secondary metabolites M-17203, UM-1 and UM-2.
- This work describes the biotransformation of F enantiomers and DHF stereoisomers in isolated male guinea pig hepatocytes. Guinea pigs were chosen with respect to similarities in F metabolism as in Man found earlier. R-F, S-F, (2R;4S)-DHF, (2S;4R)-DHF, (2S;4S)-DHF and (2R;4R)-DHF, structurally very similar compounds, served as substrates in order to observe their interaction with enzymes. Stereospecificity of the respective enzymes was studied in vitro, using hepatocytes monolayer. Chiral HPLC using R,R-ULMO column as chiral stationary phase was used for detection and quantitation of metabolites. Conclusions: Different activities and stereospecificities of the respective enzymes were observed for each substrate in primary culture of hepatocytes. Cell integrity is crucial for formation of secondary metabolites M-17203, UM-1 and UM-2. (en)
- Tato práce popisuje biotransformaci flobufenových (F) enanciomerů a dihydroflobufenových (DHF) stereoizomerů na izolovaných hepatocytech samce morčete. Morčata byla vybrána, protože byly zjištěny společné prvky F metabolizmu morčete a člověka. R-F, S-F, (2R;4S)-DHF, (2S;4S)-DHF a (2R;4R)-DHF jsou strukturně velmi podobné sloučeniny a pokud jsou podány jako substráty slouží k pozorování jejich interakce s enzymy. Stereospecifita daných enzymů byla studována in vitro. Detekce a kvantifikace metabolitů byla provedena pomocí chirálního HPLC s R,R-ULMO kolonou. Závěry: u primární kultury hepatocytů po inkubaci s různými substráty byly pozorovány odlišné aktivity a stereospecifity individuálních enzymů. (cs)
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| Title
| - The stereospecificity of flobufen metabolism in isolated guinea pig hepatocytes
- The stereospecificity of flobufen metabolism in isolated guinea pig hepatocytes (en)
- Stereospecificita metabolismu flobufenu na izolovaných hepatocytech morčete (cs)
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| skos:prefLabel
| - The stereospecificity of flobufen metabolism in isolated guinea pig hepatocytes
- The stereospecificity of flobufen metabolism in isolated guinea pig hepatocytes (en)
- Stereospecificita metabolismu flobufenu na izolovaných hepatocytech morčete (cs)
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| skos:notation
| - RIV/00216208:11150/03:00003915!RIV08-MSM-11150___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11150/03:00003915
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - stereospecificity; flobufen; metabolism; isolated; guinea; hepatocytes (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
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| http://localhost/t...ganizacniJednotka
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