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| rdf:type
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| Description
| - A laboratory-made INSTCoated fused silica capillary has been newly used for CE separation of four mixtures of proteins in sodium phosphate background electrolytes at pH 3.0 and 2.5, respectively. The obtained separation efficiencies range from 145,000 theoretical plates per meter for myoglobin to 1,216,000 m-1 for lysozyme. 49-89% of the number of theoretical plates was obtained in a commercial polyvinylalcohol coated capillary compared to the INSTCoated capillary under the same experimental conditions, 0-86% was obtained in a laboratory polyacrylamide coated capillary and only 0-6% in an uncoated fused silica capillary. The RSD values for the intra-day repeatability for an INSTCoated capillary equalled 0.1-1.0% (migration time) and 0.3-2.4% (peak area); RSD values for the inter-day repeatability in the same capillary are 0.6-1.4% (migration time) and 2.4-5.5% (peak area); RSD values for inter-day repeatability between different capillaries equalled 1.7-2.1% (migration time) and 2.8-10.9% (peak area). INSTCoated capillary has been further used for rapid determination of globin chains isolated from red blood cells. A separation of α and β chains prepared from adult blood has been completed in 3 min with peak resolution 1.3 and separation of α and Gγ chains prepared from new-born blood also at 3 min with peak resolution 3.6.
- A laboratory-made INSTCoated fused silica capillary has been newly used for CE separation of four mixtures of proteins in sodium phosphate background electrolytes at pH 3.0 and 2.5, respectively. The obtained separation efficiencies range from 145,000 theoretical plates per meter for myoglobin to 1,216,000 m-1 for lysozyme. 49-89% of the number of theoretical plates was obtained in a commercial polyvinylalcohol coated capillary compared to the INSTCoated capillary under the same experimental conditions, 0-86% was obtained in a laboratory polyacrylamide coated capillary and only 0-6% in an uncoated fused silica capillary. The RSD values for the intra-day repeatability for an INSTCoated capillary equalled 0.1-1.0% (migration time) and 0.3-2.4% (peak area); RSD values for the inter-day repeatability in the same capillary are 0.6-1.4% (migration time) and 2.4-5.5% (peak area); RSD values for inter-day repeatability between different capillaries equalled 1.7-2.1% (migration time) and 2.8-10.9% (peak area). INSTCoated capillary has been further used for rapid determination of globin chains isolated from red blood cells. A separation of α and β chains prepared from adult blood has been completed in 3 min with peak resolution 1.3 and separation of α and Gγ chains prepared from new-born blood also at 3 min with peak resolution 3.6. (en)
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| Title
| - Rapid determination of globin chains in red blood cells by capillary electrophoresis using INSTCoated fused-silica capillary
- Rapid determination of globin chains in red blood cells by capillary electrophoresis using INSTCoated fused-silica capillary (en)
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| skos:prefLabel
| - Rapid determination of globin chains in red blood cells by capillary electrophoresis using INSTCoated fused-silica capillary
- Rapid determination of globin chains in red blood cells by capillary electrophoresis using INSTCoated fused-silica capillary (en)
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| skos:notation
| - RIV/00216208:11120/14:43908205!RIV15-MSM-11120___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11120/14:43908205
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - protein separation; globin chains; clinical analysis; capillary electrophoresis; capillary coating (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Separation Science
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
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| http://linked.open...ain/vavai/riv/wos
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| issn
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| number of pages
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| http://bibframe.org/vocab/doi
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| http://localhost/t...ganizacniJednotka
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