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| Description
| - A standardisation process, already developed during the earlier European Sero-Epidemiology Network (ESEN) project, was employed with a more robust algorithm to harmonise results of pertussis serological assays performed in 12 European and non-European countries. Initially, results from each country's own assay were compared with those obtained at the reference laboratory by means of an in-house pertussis toxin (PT)-based ELISA: seven countries used in-house or commercial PT-ELISAs; the other countries used assays based on Bordetella pertussis whole cell extracts (WCE) (three countries) or on combined PT-FHA (filamentous haemagglutinin) antigenic preparations (two countries). The WCE assays, although admitted for diagnostic purposes, confirmed their low correlation with the PT-ELISAs and their results could not be used for standardisation; the PT-FHA ELISAs gave results that were suitable for standardisation in one country but unsatisfactory in the other; the use of purified PT in serological assa
- A standardisation process, already developed during the earlier European Sero-Epidemiology Network (ESEN) project, was employed with a more robust algorithm to harmonise results of pertussis serological assays performed in 12 European and non-European countries. Initially, results from each country's own assay were compared with those obtained at the reference laboratory by means of an in-house pertussis toxin (PT)-based ELISA: seven countries used in-house or commercial PT-ELISAs; the other countries used assays based on Bordetella pertussis whole cell extracts (WCE) (three countries) or on combined PT-FHA (filamentous haemagglutinin) antigenic preparations (two countries). The WCE assays, although admitted for diagnostic purposes, confirmed their low correlation with the PT-ELISAs and their results could not be used for standardisation; the PT-FHA ELISAs gave results that were suitable for standardisation in one country but unsatisfactory in the other; the use of purified PT in serological assa (en)
- Cílem této mezinárodní studie, které se účastnilo 12 států včetně ČR bylo standardizovat sérologická vyšetření protilátek proti pertusi. Použity byly metody: lokálně v laboratoři připravená metoda ELISA používající pertusový toxin (PT) komerčne vyrábená ELISA používající PT, laboratorně připravená metoda používající celobuněčný extrakt B.pertussis (WHE), ELISA PT a filament.hemagl.antigen FHA, a aglutinační metoda používající suspenzi B.pertussis. WHC metoda vykazovala nedostatečnou korelaci s PT ELISA, PT-FHA ELISA z různých laboratoří vykazovala nestandardní výsledky, Aglutinační metoda vykazovala jistou korelaci výsledků s WCE ale nikoliv s ostatními metodami. Nejlepší výsledky vykazoval ELISA s purifikovaným PT. (cs)
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| Title
| - European Sero-Epidemiology Network 2: Standardisation of Immunoassay Results for Pertussis Requires Homogeneity in the Antigenic Preparations
- European Sero-Epidemiology Network 2: Standardisation of Immunoassay Results for Pertussis Requires Homogeneity in the Antigenic Preparations (en)
- Evropská sero-epidemiologická síť 2: Standardizace sérologických metod vyšetřování pertusových protilátek vyžaduje homegenitu antigenních přípravků (cs)
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| skos:prefLabel
| - European Sero-Epidemiology Network 2: Standardisation of Immunoassay Results for Pertussis Requires Homogeneity in the Antigenic Preparations
- European Sero-Epidemiology Network 2: Standardisation of Immunoassay Results for Pertussis Requires Homogeneity in the Antigenic Preparations (en)
- Evropská sero-epidemiologická síť 2: Standardizace sérologických metod vyšetřování pertusových protilátek vyžaduje homegenitu antigenních přípravků (cs)
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| skos:notation
| - RIV/00216208:11120/08:00000699!RIV09-MSM-11120___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11120/08:00000699
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Serologic methods; pertussi; pertussis toxin; ELISA (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Griskevicius, A.
- Kafatos, G.
- Kříž, Bohumír
- Andrews, N.
- Nardone, A.
- Pebody, R.
- Schneider, F.
- Karacs, I.
- O'Flanagan, D.
- Prosenc, K.
- Chiarini, A.
- De Ory, F.
- Giammanco, A.
- Hallander, H.
- Ljungman, M.
- Marva, E.
- Taormina, S.
- Tsakris, A.
- Vrancký, R.
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| http://linked.open...ain/vavai/riv/wos
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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