About: VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation

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About: VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Employing proteomic techniques (isolation of membrane proteins, protein separation by high-resolution 2D electrophoresis, computer differential analysis, and tandem mass spectrometry) we identified two proteins with statistically significant upregulation under iron deprivation in membrane fraction of K562 cells (human erythroleukemia), i.e., aldolase A (ALDA) and voltage-dependent anion channel 2 (VDAC2). The increased expression of aldolase A and VDAC2 under iron deprivation was also confirmed by western blot analysis. This is the first time when the control of aldolase A and VDAC2 levels by iron status of the cell is demonstrated. Employing proteomic techniques (isolation of membrane proteins, protein separation by high-resolution 2D electrophoresis, computer differential analysis, and tandem mass spectrometry) we identified two proteins with statistically significant upregulation under iron deprivation in membrane fraction of K562 cells (human erythroleukemia), i.e., aldolase A (ALDA) and voltage-dependent anion channel 2 (VDAC2). The increased expression of aldolase A and VDAC2 under iron deprivation was also confirmed by western blot analysis. This is the first time when the control of aldolase A and VDAC2 levels by iron status of the cell is demonstrated. (en) Použitím proteomických technik (izolace membránových proteinů, separace proteinů pomocí 2D elektroforézy s vysokou rozlišovací schopností, počítačová diferenciální analýza a tandemová hmotnostní spektrometrie) jsme identifikovali dva proteiny se statisticky významnou upregulací při deprivaci železa v membránové frakci buněk K562 (lidská erytroleukémie): aldolázu A (ALDA) a voltage-dependent anion channel 2 (VDAC2). Zvýšená exprese aldolázy A a VDAC2 při deprivaci železa byla také potvrzena pomocí western blot analýzy. Poprvé jsme demonstrovali, že exprese aldolázy A a VDAC2 může být kontrolována hladinou železa. (cs)
Title	VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation VDAC2 a aldoláza A identifikovány jako membránové proteiny buněk K562 se zvýšenou expresí při deprivaci železa (cs) VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation (en)
skos:prefLabel	VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation VDAC2 a aldoláza A identifikovány jako membránové proteiny buněk K562 se zvýšenou expresí při deprivaci železa (cs) VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation (en)
skos:notation	RIV/00216208:11120/08:00000522!RIV09-AV0-11120___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA5052402), Z(AV0Z50200510), Z(AV0Z50520701), Z(MSM0021620814)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Neubauerová, Jitka Kovář, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	402377
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/08:00000522
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Iron deprivation; K562 cells; Membrane proteins; Aldolase A; VDAC2 (en)
http://linked.open.../riv/klicoveSlovo	Membrane proteins Aldolase A Iron deprivation VDAC2 K562 cells
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[37837CF6F44B]
http://linked.open...i/riv/nazevZdroje	Molecular and Cellular Biochemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Transport of low-molecular-mass iron into human cells: identification of transport molecules
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	311
http://linked.open...iv/tvurceVysledku	Kovář, Jan Neubauerová, Jitka Man, P. Vališ, K. Pompach, P. Vohradský, J.
http://linked.open...ain/vavai/riv/wos	000254204500025
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Establishing of the Biotechnology Institute ASCR Prevention, diagnostics and therapy of diabetes mellitus, metabolic and endocrine damage of organism
issn	0300-8177
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	11120
is http://linked.open...avai/riv/vysledek of	VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation

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