About: CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers     Goto   Sponge   NotDistinct   Permalink

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  • Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, phenotype, and distribution. Single-cell suspensions from normal mouse corneas were also prepared and analyzed by flow cytometry. After short-term culture of corneal stromal explants, nonadherent cells were harvested and cytospins were prepared and stained for different markers. Combined staining for F-actin and leukocyte differentiation markers clearly showed that the corneal stroma contains a population of CD45(+) resident bone marrow-derived cells, whereas most cells were CD45-F-actin(+) keratocytes. A significant proportion (two thirds) of CD45(+) cells in the normal corneal stroma expressed CD34(+), whereas no CD45(-) cells (i.e., kera
  • Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, phenotype, and distribution. Single-cell suspensions from normal mouse corneas were also prepared and analyzed by flow cytometry. After short-term culture of corneal stromal explants, nonadherent cells were harvested and cytospins were prepared and stained for different markers. Combined staining for F-actin and leukocyte differentiation markers clearly showed that the corneal stroma contains a population of CD45(+) resident bone marrow-derived cells, whereas most cells were CD45-F-actin(+) keratocytes. A significant proportion (two thirds) of CD45(+) cells in the normal corneal stroma expressed CD34(+), whereas no CD45(-) cells (i.e., kera (en)
Title
  • CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers
  • CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers (en)
skos:prefLabel
  • CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers
  • CD34(+) corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers (en)
skos:notation
  • RIV/00216208:11120/05:00003842!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 514570
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11120/05:00003842
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cornea; CD34; hemopoietic stem cell; dendritic cell; leukocytes (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [28A2E4986DEF]
http://linked.open...i/riv/nazevZdroje
  • Stem Cells
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 23
http://linked.open...iv/tvurceVysledku
  • Sosnová, Magdaléna
http://linked.open...ain/vavai/riv/wos
  • 000228161800009
issn
  • 1066-5099
number of pages
http://bibframe.org/vocab/doi
  • 10.1634/stemcells.2004-0291
http://localhost/t...ganizacniJednotka
  • 11120
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