About: Cell Adhesion on polytetrafluoroethylene modified by UV-irradiation in an ammonia atmosphere

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About: Cell Adhesion on polytetrafluoroethylene modified by UV-irradiation in an ammonia atmosphere Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1002/jbm.a.10043
Description	We report on the modification of polytetrafluoroethylene (PTFE) by exposure to the ultraviolet (UV) light of a Xe-2-excimer lamp at a wavelength of 172 nm in an ammonia atmosphere. Typical treatment times were up to 30 min. Subsequently, the samples were grafted with the amino acid alanine from an aqueous solution. The samples were characterized by means of optical transmission spectroscopy, laser-induced fluorescence and contact-angle measurements. We studied the adhesion of rat aortic smooth muscle cells (SMC) and mouse fibroblasts (3T3 cells) to the modified polymer samples using an in vitro technique, where the population density and spreadout of adhering cells is determined 24 h after seeding by image analysis. For both cell types the exposure of PTFE to UV-Iight in an ammonia atmosphere resulted in a significant increase in the number of adhering cells and in the size of their spreading area. The grafting with alanine enhanced this effect. Additional experiments with human endothelial cells (HE We report on the modification of polytetrafluoroethylene (PTFE) by exposure to the ultraviolet (UV) light of a Xe-2-excimer lamp at a wavelength of 172 nm in an ammonia atmosphere. Typical treatment times were up to 30 min. Subsequently, the samples were grafted with the amino acid alanine from an aqueous solution. The samples were characterized by means of optical transmission spectroscopy, laser-induced fluorescence and contact-angle measurements. We studied the adhesion of rat aortic smooth muscle cells (SMC) and mouse fibroblasts (3T3 cells) to the modified polymer samples using an in vitro technique, where the population density and spreadout of adhering cells is determined 24 h after seeding by image analysis. For both cell types the exposure of PTFE to UV-Iight in an ammonia atmosphere resulted in a significant increase in the number of adhering cells and in the size of their spreading area. The grafting with alanine enhanced this effect. Additional experiments with human endothelial cells (HE (en)
Title	Cell Adhesion on polytetrafluoroethylene modified by UV-irradiation in an ammonia atmosphere Cell Adhesion on polytetrafluoroethylene modified by UV-irradiation in an ammonia atmosphere (en)
skos:prefLabel	Cell Adhesion on polytetrafluoroethylene modified by UV-irradiation in an ammonia atmosphere Cell Adhesion on polytetrafluoroethylene modified by UV-irradiation in an ammonia atmosphere (en)
skos:notation	RIV/00216208:11120/03:00003837!RIV12-MSM-11120___
http://linked.open...avai/riv/aktivita	N P Z
http://linked.open...avai/riv/aktivity	N, P(IAA5011301), Z(AV0Z5011922)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Dvořánková, Barbora
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / 3. lékařská fakulta
http://linked.open...dnocenehoVysledku	600504
http://linked.open...ai/riv/idVysledku	RIV/00216208:11120/03:00003837
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cell adhesion; UV-modified polymer; amino acid grafting; vascular smooth muscle cells; fibroblasts; endothelial cells (en)
http://linked.open.../riv/klicoveSlovo	fibroblasts cell adhesion endothelial cells vascular smooth muscle cells UV-modified polymer amino acid grafting
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[5FE140658C56]
http://linked.open...i/riv/nazevZdroje	Journal of Biomedical Materials Research
http://linked.open...in/vavai/riv/obor	FP
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Vascular cells on synthetic polymers with micro-patterned srufaces
http://linked.open...UplatneniVysledku	2003
http://linked.open...v/svazekPeriodika	67A
http://linked.open...iv/tvurceVysledku	Dvořánková, Barbora Heitz, J.
http://linked.open...ain/vavai/riv/wos	000185760300016
http://linked.open...n/vavai/riv/zamer	Investigation of normal and pathological physiology of animals - the clarification of the physiological mechanisms operating in humans in health and disease
issn	0021-9304
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jbm.a.10043
http://localhost/t...ganizacniJednotka	11120

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