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Description
  • The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.
  • The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue. (en)
Title
  • Our experiences with B cell clonality analysis using multiplex PCR amplification in paraffin-embedded tissue
  • Our experiences with B cell clonality analysis using multiplex PCR amplification in paraffin-embedded tissue (en)
skos:prefLabel
  • Our experiences with B cell clonality analysis using multiplex PCR amplification in paraffin-embedded tissue
  • Our experiences with B cell clonality analysis using multiplex PCR amplification in paraffin-embedded tissue (en)
skos:notation
  • RIV/00216208:11110/14:10285045!RIV15-MSM-11110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 35355
http://linked.open...ai/riv/idVysledku
  • RIV/00216208:11110/14:10285045
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • heteroduplex; paraffin-embedded tissue; IgH; PCR; monoclonality (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • SK - Slovenská republika
http://linked.open...ontrolniKodProRIV
  • [478952C696A8]
http://linked.open...i/riv/nazevZdroje
  • Acta Medica Martiniana
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 14
http://linked.open...iv/tvurceVysledku
  • Lísová, Soňa
  • Staněk, Libor
  • Tvrdík, Daniel
issn
  • 1335-8421
number of pages
http://bibframe.org/vocab/doi
  • 10.2478/acm-2014-0001
http://localhost/t...ganizacniJednotka
  • 11110
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