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| Description
| - The multifunctional factors Imp-alpha and Imp-beta are involved in nuclear protein import, mitotic spindle dynamics, and nuclear membrane formation. Furthermore, each of the three members of the Imp-alpha family exerts distinct tasks during development. In Drosophila melanogaster, the imp-alpha 2 gene is critical during oogenesis for ring canal assembly; specific mutations, which allow oogenesis to proceed normally, were found to block early embryonic mitosis. Here, we show that imp-alpha 2 and imp-beta genetically interact during early embryonic development, and we characterize the pattern of defects affecting mitosis in embryos laid by heterozygous imp-alpha 2(D14) and imp-beta(KetRE34) females. Embryonic development is arrested in these embryos but is unaffected in combinations between imp-beta(KetRE34) and null mutations in imp-alpha 1 or imp-alpha 3. Furthermore, the imp-alpha 2(D14)/imp-beta(KetRE34) interaction could only be rescued by an imp-alpha 2 transgene, albeit not imp-alpha 1 or imp-alpha 3, showing the exclusive imp-alpha 2 function with imp-beta. Use of transgenes carrying modifications in the major Imp-alpha 2 domains showed the critical requirement of the nuclear localization signal binding (NLSB) site in this process. In the mutant embryos, we found metaphase-arrested mitoses made of enlarged spindles, suggesting an unrestrained activity of factors promoting spindle assembly. In accordance with this, we found that Imp-beta(KetRE34) and Imp-beta(KetD) bind a high level of RanGTP/GDP, and a deletion decreasing RanGTP level suppresses the imp-beta(KetRE34) phenotype. These data suggest that a fine balance among Imp-alpha 2, Imp-beta, RanGTP, and the NLS cargos is critical for mitotic progression during early embryonic development.
- The multifunctional factors Imp-alpha and Imp-beta are involved in nuclear protein import, mitotic spindle dynamics, and nuclear membrane formation. Furthermore, each of the three members of the Imp-alpha family exerts distinct tasks during development. In Drosophila melanogaster, the imp-alpha 2 gene is critical during oogenesis for ring canal assembly; specific mutations, which allow oogenesis to proceed normally, were found to block early embryonic mitosis. Here, we show that imp-alpha 2 and imp-beta genetically interact during early embryonic development, and we characterize the pattern of defects affecting mitosis in embryos laid by heterozygous imp-alpha 2(D14) and imp-beta(KetRE34) females. Embryonic development is arrested in these embryos but is unaffected in combinations between imp-beta(KetRE34) and null mutations in imp-alpha 1 or imp-alpha 3. Furthermore, the imp-alpha 2(D14)/imp-beta(KetRE34) interaction could only be rescued by an imp-alpha 2 transgene, albeit not imp-alpha 1 or imp-alpha 3, showing the exclusive imp-alpha 2 function with imp-beta. Use of transgenes carrying modifications in the major Imp-alpha 2 domains showed the critical requirement of the nuclear localization signal binding (NLSB) site in this process. In the mutant embryos, we found metaphase-arrested mitoses made of enlarged spindles, suggesting an unrestrained activity of factors promoting spindle assembly. In accordance with this, we found that Imp-beta(KetRE34) and Imp-beta(KetD) bind a high level of RanGTP/GDP, and a deletion decreasing RanGTP level suppresses the imp-beta(KetRE34) phenotype. These data suggest that a fine balance among Imp-alpha 2, Imp-beta, RanGTP, and the NLS cargos is critical for mitotic progression during early embryonic development. (en)
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| Title
| - Specific Cooperation Between Imp-alpha 2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions
- Specific Cooperation Between Imp-alpha 2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions (en)
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| skos:prefLabel
| - Specific Cooperation Between Imp-alpha 2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions
- Specific Cooperation Between Imp-alpha 2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions (en)
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| skos:notation
| - RIV/00216208:11110/12:12788!RIV13-GA0-11110___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216208:11110/12:12788
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Drosophila; Importins; genetic interaction; mitosis; spindle formation (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - G3-GENES GENOMES GENETICS
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Mechler, Bernard
- Eichhorn, Tolga
- Gorjanacz, Matyas
- Kallakuri, Sowjanya
- Kiss, István
- Szlanka, Tamás
- Török, Istvan
- Virágh, Erika
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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