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Description
| - Her-2/PI3K/Akt kinázová signální dráha může hrát specifickou pro-apoptotickou roli u některých typů buněk díky pozitivní regulaci exprese apoptotických receptorů TRAIL-R1 a TRAIL-R2. Potlačení Her2 signalizace prostřednictvím trastuzumabu (Herceptin) vedlo ke snížené odpovědi buněk BT474 odvozených od karcinomu prsu na navození apoptózy ligandem TRAIL. Tento efekt byl pozorován také při inhibici PI3K/Akt kinázové nikoli MAPK/ERK kinázové dráhy. U buněk BT474 inhibice Her-2/PI3K/Akt kinázové signalizace vedla k redukci exprese receptorů pro TRAIL a některých apoptotických procesů navozených TRAILem jako je aktivace kaspázy-8, štěpení proteinu Bid, pokles mitochondriálního potenciálu a štěpení PARP nikoli však aktivace kaspázy-9. (cs)
- We show that HER2/PI3K/Akt pathway may play a specific pro-apoptotic role in certain cell type by inducing TRAIL-R1 and -R2 expression and thereby enhancing responsiveness to TRAIL. HER2 downregulation by trastuzumab decreased susceptibility of BT474 breast cancer cells to TRAIL-induced apoptosis. This decrease was also observed after exogenous inhibition of PI3-K/Akt kinase, but not MAPK/ERK kinase. In BT474 cells, inhibition of the HER2/PI3K/Akt pathway resulted in downregulation of the pro-apoptotic receptors TRAIL-R1 and TRAIL-R2. TRAIL-induced caspase-8 activation, Bid processing, drop of DeltaPsi(m), and poly ADP-ribose polymerase (PARP) cleavage but not in caspase-9 activation were inhibited in HER2/PI3K/Akt-suppressed BT474 cells.
- We show that HER2/PI3K/Akt pathway may play a specific pro-apoptotic role in certain cell type by inducing TRAIL-R1 and -R2 expression and thereby enhancing responsiveness to TRAIL. HER2 downregulation by trastuzumab decreased susceptibility of BT474 breast cancer cells to TRAIL-induced apoptosis. This decrease was also observed after exogenous inhibition of PI3-K/Akt kinase, but not MAPK/ERK kinase. In BT474 cells, inhibition of the HER2/PI3K/Akt pathway resulted in downregulation of the pro-apoptotic receptors TRAIL-R1 and TRAIL-R2. TRAIL-induced caspase-8 activation, Bid processing, drop of DeltaPsi(m), and poly ADP-ribose polymerase (PARP) cleavage but not in caspase-9 activation were inhibited in HER2/PI3K/Akt-suppressed BT474 cells. (en)
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Title
| - HER2 signaling downregulation by trastuzumab and suppression of the PI3K/Akt pathway
- Potlačení Her-2 signalizace trastuzumabem a inhibice PI3K/Akt kinázové dráhy (cs)
- HER2 signaling downregulation by trastuzumab and suppression of the PI3K/Akt pathway (en)
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skos:prefLabel
| - HER2 signaling downregulation by trastuzumab and suppression of the PI3K/Akt pathway
- Potlačení Her-2 signalizace trastuzumabem a inhibice PI3K/Akt kinázové dráhy (cs)
- HER2 signaling downregulation by trastuzumab and suppression of the PI3K/Akt pathway (en)
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skos:notation
| - RIV/00209805:_____/05:#0000017!RIV06-MZ0-00209805
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00209805:_____/05:#0000017
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - TRAIL, Apoptosis, HER2/neu, Trastuzumab, PI3K, Breast cancer (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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