About: Identification of Lactobacillus spp. isolated from dental caries of children

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Aim. To isolate and characterize Lactobacillus spp. inhabiting dental caries of children (up to age of 10). Methods. A total of 33 lactobacilli were isolated from 26 cases in period August to December 2005. Caries samples obtained from individual patients were cultivated in 5 % CO2 atmosphere on MRS and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization of the isolates. Results. Evaluation of biochemical test results assigned 2 strains as L. acidophilus, 2 as L. casei, 2 as L. cellobiosus, 3 as L. fermentum, 1 as L. paracasei, 1 as L. plantarum, 5 as L. rhamnosus and 4 as L. salivarius, remaining strains (13) were not identified only to the species level. (GTG)5-PCR fingerprinting grouped the analysed strains into a few clusters in nearly full agreement with biotype results and clarified taxonomic positio Aim. To isolate and characterize Lactobacillus spp. inhabiting dental caries of children (up to age of 10). Methods. A total of 33 lactobacilli were isolated from 26 cases in period August to December 2005. Caries samples obtained from individual patients were cultivated in 5 % CO2 atmosphere on MRS and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization of the isolates. Results. Evaluation of biochemical test results assigned 2 strains as L. acidophilus, 2 as L. casei, 2 as L. cellobiosus, 3 as L. fermentum, 1 as L. paracasei, 1 as L. plantarum, 5 as L. rhamnosus and 4 as L. salivarius, remaining strains (13) were not identified only to the species level. (GTG)5-PCR fingerprinting grouped the analysed strains into a few clusters in nearly full agreement with biotype results and clarified taxonomic positio (en)
Title	Identification of Lactobacillus spp. isolated from dental caries of children Identification of Lactobacillus spp. isolated from dental caries of children (en)
skos:prefLabel	Identification of Lactobacillus spp. isolated from dental caries of children Identification of Lactobacillus spp. isolated from dental caries of children (en)
skos:notation	RIV/00159816:_____/06:#0000162!RIV10-MSM-00159816
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M0528), Z(MSM0021622416)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Sedláček, Ivo Švec, Pavel Kukletová, Martina Kuklová, Jarmila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice u sv.Anny v Brně
http://linked.open...dnocenehoVysledku	478722
http://linked.open...ai/riv/idVysledku	RIV/00159816:_____/06:#0000162
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Lactobacillus, identification, taxonomy, dental plaque (en)
http://linked.open.../riv/klicoveSlovo	Lactobacillus identification taxonomy dental plaque
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[53509379C363]
http://linked.open...i/riv/nazevZdroje	European Archives of paediatric dentistry
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Dental research centre
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Sedláček, Ivo Švec, Pavel Kukletová, Martina Kuklová, Jarmila Žáčková, Lenka
http://linked.open...n/vavai/riv/zamer	Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
issn	1818-6300
number of pages	1 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Identification of Lactobacillus spp. isolated from dental caries of children Identification of Lactobacillus spp. isolated from dental caries of children

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