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| Description
| - This study aims to investigate the effect of silybin on liver expression and activity of rat CYP2C6 under mild liver steatosis. This cytochrome P450 enzyme is considered to be a counterpart of human CYP2C9, which metabolizes commonly prescribed drugs, such as ibuprofen, diclofenac, or warfarin. Male hereditary hypertriglyceridemic rats were fed: 1) standard laboratory diet (STD), 2) high cholesterol diet, 3) high cholesterol diet with silybin for 21 days. Expression of cytochrome P450 2C6 was measured in liver using real-time PCR (at mRNA level) and Western blotting (at protein level). Formation of diclofenac metabolite (typical marker substrate of CYP2C6 enzyme activity) was analyzed using HPLC with UV detection. Silybin in hereditary hypertriglyceridemic rats on HCD diet significantly increased activity of CYP2C6 and its expression on mRNA level. Expression of CYP2C6 on protein level was non-significantly affected by silybin consumption. Our results suggest that CYP2C6 is up-regulated by silybin in hereditary hypertriglyceridemic rats on high cholesterol diet. Because cytochrome P450 2C6 is considered to be a counterpart of human CYP2C9, the results obtained open the possibility that in human silybin may affect the metabolism of drugs metabolized by this cytochrome P450. Further studies are needed to elucidate the effects of silybin on CYP2C9 in humans suffering from metabolic syndrome.
- This study aims to investigate the effect of silybin on liver expression and activity of rat CYP2C6 under mild liver steatosis. This cytochrome P450 enzyme is considered to be a counterpart of human CYP2C9, which metabolizes commonly prescribed drugs, such as ibuprofen, diclofenac, or warfarin. Male hereditary hypertriglyceridemic rats were fed: 1) standard laboratory diet (STD), 2) high cholesterol diet, 3) high cholesterol diet with silybin for 21 days. Expression of cytochrome P450 2C6 was measured in liver using real-time PCR (at mRNA level) and Western blotting (at protein level). Formation of diclofenac metabolite (typical marker substrate of CYP2C6 enzyme activity) was analyzed using HPLC with UV detection. Silybin in hereditary hypertriglyceridemic rats on HCD diet significantly increased activity of CYP2C6 and its expression on mRNA level. Expression of CYP2C6 on protein level was non-significantly affected by silybin consumption. Our results suggest that CYP2C6 is up-regulated by silybin in hereditary hypertriglyceridemic rats on high cholesterol diet. Because cytochrome P450 2C6 is considered to be a counterpart of human CYP2C9, the results obtained open the possibility that in human silybin may affect the metabolism of drugs metabolized by this cytochrome P450. Further studies are needed to elucidate the effects of silybin on CYP2C9 in humans suffering from metabolic syndrome. (en)
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| Title
| - Silybin affects the liver microsomal CYP2C6 in HHTg rats
- Silybin affects the liver microsomal CYP2C6 in HHTg rats (en)
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| skos:prefLabel
| - Silybin affects the liver microsomal CYP2C6 in HHTg rats
- Silybin affects the liver microsomal CYP2C6 in HHTg rats (en)
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| skos:notation
| - RIV/00098892:_____/13:#0000416!RIV14-MZ0-00098892
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00098892:_____/13:#0000416
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Silybin, liver, microsomal, CYP2C6, HHTg, rats (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Biomedical Papers-Olomouc
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Strojil, Jan
- Anzenbacher, Pavel
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| issn
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| number of pages
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